Antibacterial activity of fresh and processed red muscadine juice and the role of their polar compounds on Escherichia coli O157:H7

The objectives of this research were to show the anti-Escherichia coli O157:H7 effect of fresh (FRMJ) and processed red muscadine (Vitis rotundifolia) juice (PRMJ) and to discern the active compounds responsible for anti-E. coli O157:H7. Polar and phenolic compounds of FRMJ and PRMJ were analysed by...

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Bibliographic Details
Published in:Journal of applied microbiology 2009-08, Vol.107 (2), p.533-539
Main Authors: Kim, T.J, Silva, J.L, Jung, Y.S
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
antimicrobial activity
Beverages - microbiology
Biological and medical sciences
Chromatography, High Pressure Liquid
Colony Count, Microbial
Dicarboxylic Acids - pharmacology
Escherichia coli
Escherichia coli O157 - drug effects
Escherichia coli O157 - growth & development
Escherichia coli O157:H7
Food Microbiology
Food Preservation - methods
Fundamental and applied biological sciences. Psychology
Malates
malic acid
Microbiology
polar and polyphenol fractions
red muscadine juice
sugars
tannic acid
tannins
Tannins - pharmacology
tartaric acid
Tartrates
Vitaceae
Vitis - chemistry
Vitis - microbiology
Vitis rotundifolia
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Summary:The objectives of this research were to show the anti-Escherichia coli O157:H7 effect of fresh (FRMJ) and processed red muscadine (Vitis rotundifolia) juice (PRMJ) and to discern the active compounds responsible for anti-E. coli O157:H7. Polar and phenolic compounds of FRMJ and PRMJ were analysed by high-performance liquid chromatography. Antibacterial activity of FRMJ, PRMJ, their polar and polyphenol fractions, individual synthetic acids and their mixture with or without sugars were investigated on E. coli O157:H7. FRMJ and PRMJ inactivated (P [less-than or equal to] 0·05) 5-log cocktail cells of E. coli O157:H7 within 4 h at 37°C. Polar fractions that contained malic, tartaric and tannic acids showed strong antimicrobial activity (P [less-than or equal to] 0·05) against E. coli O157:H7. Tannic acid among the synthetic acids showed the highest antimicrobial activity against E. coli O157:H7. FRMJ, PRMJ and their polar compounds showed strong anti-E. coli O157:H7 activity. Earlier findings have failed to show any anti-E. coli O157:H7 effect of grape juice without adding preservatives. Our findings show that red muscadine juice has natural antibacterial substances and suggest that these can be used as active antimicrobial ingredients against E. coli O157:H7 in nonalcoholic beverages.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2009.04239.x