Selected nondigestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono-cultures in vitro

• Published in: Journal of applied microbiology 2009-03, Vol.106 (3), p.932-940
• Main Authors: Rurangwa, E, Laranja, J.L, Van Houdt, R, Delaedt, Y, Geraylou, Z, Van de Wiele, T, Van Loo, J, Van Craeyveld, V, Courtin, C.M, Delcour, J.A, Ollevier, F
• Format: Article
• Language: English
• Subjects: Acetic Acid - metabolism; Animals; Bacillus subtilis; Bacillus subtilis - growth & development; Bacteria - growth & development; Bacteria - metabolism; Bacteriological Techniques; beta-Glucans - metabolism; Biological and medical sciences; Carbohydrate Metabolism; Carnobacterium - growth & development; Carnobacterium piscicola; Colony Count, Microbial; Culture Media; fish; Fishes - microbiology; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; growth; in vitro; Inulin - metabolism; Lactobacillus - growth & development; Lactobacillus delbrueckii; Lactobacillus plantarum; Microbiology; nondigestible carbohydrates; Oligosaccharides - metabolism; Prebiotics; Probiotics; short chain fatty acid
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2008.04034.x

To search for nondigestible but fermentable (NDF) carbohydrates and prebiotics with a potency to promote the growth of selected bacteria in vitro. The growth of three reference bacteria strains Bacillus subtilis LMG 7135T, Carnobacterium piscicola LMG 9839, Lactobacillus plantarum LMG 9211 and one candidate probiotic bacteria Lactobacillus delbrueckii subsp. lactis was investigated over a minimum period of 48 h in the presence of β-glucan, xylo-oligosaccharide, arabinoxylo-oligosaccharide, inulin, oligofructose and glucose. Besides the capability to grow on inulin and oligofructose containing media, a distinct high growth in β-glucan based substrates and a low growth in (arabino)xylooligosaccharide containing media were evident for most bacteria tested. With the exception of B. subtilis and L. plantarum, other bacteria grew equally well or even better on different substrates than on glucose. The fermentation of studied carbohydrates by these micro-organisms was dominated by the production of acetic acid as the main short chain fatty acid. Selected bacteria are able to ferment and grow on NDF and prebiotic carbohydrates but in a substrate dependent manner. This study delivers a first screening of which NDF or prebiotic carbohydrates are the most promising for aquaculture feed supplementations.