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P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions

It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X 1–7 and P2Y 1–14 receptors; however, the pres...

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Published in:Naunyn-Schmiedeberg's archives of pharmacology 2009-06, Vol.379 (6), p.541-549
Main Authors: Musa, Hanny, Tellez, James O., Chandler, Natalie J., Greener, Ian D., Mączewski, Michał, Mackiewicz, Urszula, Beresewicz, Andrzej, Molenaar, Peter, Boyett, Mark R., Dobrzynski, Halina
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Language:English
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Summary:It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X 1–7 and P2Y 1–14 receptors; however, the presence of P2X and P2Y receptor subtypes in strategic cardiac locations such as the sinoatrial node has not been determined. An understanding of P2X and P2Y receptor localization would facilitate investigation of purine receptor function in the heart. Therefore, we used quantitative PCR and in situ hybridization to measure the expression of mRNA of all known purine receptors in rat left ventricle, right atrium and sinoatrial node (SAN), and human right atrium and SAN. Expression of mRNA for all the cloned P2 receptors was observed in the ventricles, atria, and SAN of the rat. However, their abundance varied in different regions of the heart. P2X 5 was the most abundant of the P2X receptors in all three regions of the rat heart. In rat left ventricle, P2Y 1 , P2Y 2 , and P2Y 14 mRNA levels were highest for P2Y receptors, while in right atrium and SAN, P2Y 2 and P2Y 14 levels were highest, respectively. We extended these studies to investigate P2X 4 receptor mRNA in heart from rats with coronary artery ligation-induced heart failure. P2X 4 receptor mRNA was upregulated by 93% in SAN ( P  
ISSN:0028-1298
1432-1912
DOI:10.1007/s00210-009-0403-2