Loading…
P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions
It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X 1–7 and P2Y 1–14 receptors; however, the pres...
Saved in:
| Published in: | Naunyn-Schmiedeberg's archives of pharmacology 2009-06, Vol.379 (6), p.541-549 |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83 |
| container_end_page | 549 |
| container_issue | 6 |
| container_start_page | 541 |
| container_title | Naunyn-Schmiedeberg's archives of pharmacology |
| container_volume | 379 |
| creator | Musa, Hanny Tellez, James O. Chandler, Natalie J. Greener, Ian D. Mączewski, Michał Mackiewicz, Urszula Beresewicz, Andrzej Molenaar, Peter Boyett, Mark R. Dobrzynski, Halina |
| description | It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X
1–7
and P2Y
1–14
receptors; however, the presence of P2X and P2Y receptor subtypes in strategic cardiac locations such as the sinoatrial node has not been determined. An understanding of P2X and P2Y receptor localization would facilitate investigation of purine receptor function in the heart. Therefore, we used quantitative PCR and in situ hybridization to measure the expression of mRNA of all known purine receptors in rat left ventricle, right atrium and sinoatrial node (SAN), and human right atrium and SAN. Expression of mRNA for all the cloned P2 receptors was observed in the ventricles, atria, and SAN of the rat. However, their abundance varied in different regions of the heart. P2X
5
was the most abundant of the P2X receptors in all three regions of the rat heart. In rat left ventricle, P2Y
1
, P2Y
2
, and P2Y
14
mRNA levels were highest for P2Y receptors, while in right atrium and SAN, P2Y
2
and P2Y
14
levels were highest, respectively. We extended these studies to investigate P2X
4
receptor mRNA in heart from rats with coronary artery ligation-induced heart failure. P2X
4
receptor mRNA was upregulated by 93% in SAN (
P
|
| doi_str_mv | 10.1007/s00210-009-0403-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733327409</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733327409</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83</originalsourceid><addsrcrecordid>eNp9kLtOwzAUhi0EoqXwACzIG1Pg2M7NY1VxkypAqMyWYzttqsQOdjLw9rikEhtn8fBfzvGH0DWBOwJQ3AcASiAB4AmkwBJ6guYkZTQhnNBTNI9ymRDKyxm6CGEPADnJsnM0I5yyNC_5HG3eKe5H31jjt43C3ijTD87j7uN1iRuLvRywtBrvxk5aHBrr5OAb2WLrtPlV3LAzHu-M9EOMbxtnwyU6q2UbzNXxXaDPx4fN6jlZvz29rJbrRKXAh4TmRVZpHW-STJdM0aoimuYpi6Og5opVuVQpKWqW6ZIWBQfFdUVSSbjmdckW6Hbq7b37Gk0YRNcEZdpWWuPGIIpYRIu4KzrJ5FTeheBNLXrfdNJ_CwLiwFJMLEVkKQ4sBY2Zm2P7WHVG_yWO8KKBToYQJbs1Xuzd6G388T-tPz3xffc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733327409</pqid></control><display><type>article</type><title>P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions</title><source>Springer Nature</source><creator>Musa, Hanny ; Tellez, James O. ; Chandler, Natalie J. ; Greener, Ian D. ; Mączewski, Michał ; Mackiewicz, Urszula ; Beresewicz, Andrzej ; Molenaar, Peter ; Boyett, Mark R. ; Dobrzynski, Halina</creator><creatorcontrib>Musa, Hanny ; Tellez, James O. ; Chandler, Natalie J. ; Greener, Ian D. ; Mączewski, Michał ; Mackiewicz, Urszula ; Beresewicz, Andrzej ; Molenaar, Peter ; Boyett, Mark R. ; Dobrzynski, Halina</creatorcontrib><description>It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X
1–7
and P2Y
1–14
receptors; however, the presence of P2X and P2Y receptor subtypes in strategic cardiac locations such as the sinoatrial node has not been determined. An understanding of P2X and P2Y receptor localization would facilitate investigation of purine receptor function in the heart. Therefore, we used quantitative PCR and in situ hybridization to measure the expression of mRNA of all known purine receptors in rat left ventricle, right atrium and sinoatrial node (SAN), and human right atrium and SAN. Expression of mRNA for all the cloned P2 receptors was observed in the ventricles, atria, and SAN of the rat. However, their abundance varied in different regions of the heart. P2X
5
was the most abundant of the P2X receptors in all three regions of the rat heart. In rat left ventricle, P2Y
1
, P2Y
2
, and P2Y
14
mRNA levels were highest for P2Y receptors, while in right atrium and SAN, P2Y
2
and P2Y
14
levels were highest, respectively. We extended these studies to investigate P2X
4
receptor mRNA in heart from rats with coronary artery ligation-induced heart failure. P2X
4
receptor mRNA was upregulated by 93% in SAN (
P
< 0.05), while a trend towards an increase was also observed in the right atrium and left ventricle (not significant). Thus, P2X
4
-mediated effects might be modulated in heart failure. mRNA for P2X
4–7
and P2Y
1,2,4,6,12–14
, but not P2X
2,3
and P2Y
11
, was detected in human right atrium and SAN. In addition, mRNA for P2X
1
was detected in human SAN but not human right atrium. In human right atrium and SAN, P2X
4
and P2X
7
mRNA was the highest for P2X receptors. P2Y
1
and P2Y
2
mRNA were the most abundant for P2Y receptors in the right atrium, while P2Y
1
, P2Y
2
, and P2Y
14
were the most abundant P2Y receptor subtypes in human SAN. This study shows a widespread distribution of P2 receptor mRNA in rat heart tissues but a more restricted presence and distribution of P2 receptor mRNA in human atrium and SAN. This study provides further direction for the elucidation of P2 receptor modulation of heart rate and contractility.</description><identifier>ISSN: 0028-1298</identifier><identifier>EISSN: 1432-1912</identifier><identifier>DOI: 10.1007/s00210-009-0403-2</identifier><identifier>PMID: 19234689</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Adult ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Female ; Humans ; Male ; Middle Aged ; Myocardium - chemistry ; Myocardium - metabolism ; Neurosciences ; Original Aricle ; Pharmacology/Toxicology ; Rats ; Rats, Wistar ; Receptors, Purinergic P2 - analysis ; Receptors, Purinergic P2 - metabolism ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; Sinoatrial Node - chemistry ; Sinoatrial Node - metabolism</subject><ispartof>Naunyn-Schmiedeberg's archives of pharmacology, 2009-06, Vol.379 (6), p.541-549</ispartof><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83</citedby><cites>FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19234689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Musa, Hanny</creatorcontrib><creatorcontrib>Tellez, James O.</creatorcontrib><creatorcontrib>Chandler, Natalie J.</creatorcontrib><creatorcontrib>Greener, Ian D.</creatorcontrib><creatorcontrib>Mączewski, Michał</creatorcontrib><creatorcontrib>Mackiewicz, Urszula</creatorcontrib><creatorcontrib>Beresewicz, Andrzej</creatorcontrib><creatorcontrib>Molenaar, Peter</creatorcontrib><creatorcontrib>Boyett, Mark R.</creatorcontrib><creatorcontrib>Dobrzynski, Halina</creatorcontrib><title>P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions</title><title>Naunyn-Schmiedeberg's archives of pharmacology</title><addtitle>Naunyn-Schmied Arch Pharmacol</addtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><description>It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X
1–7
and P2Y
1–14
receptors; however, the presence of P2X and P2Y receptor subtypes in strategic cardiac locations such as the sinoatrial node has not been determined. An understanding of P2X and P2Y receptor localization would facilitate investigation of purine receptor function in the heart. Therefore, we used quantitative PCR and in situ hybridization to measure the expression of mRNA of all known purine receptors in rat left ventricle, right atrium and sinoatrial node (SAN), and human right atrium and SAN. Expression of mRNA for all the cloned P2 receptors was observed in the ventricles, atria, and SAN of the rat. However, their abundance varied in different regions of the heart. P2X
5
was the most abundant of the P2X receptors in all three regions of the rat heart. In rat left ventricle, P2Y
1
, P2Y
2
, and P2Y
14
mRNA levels were highest for P2Y receptors, while in right atrium and SAN, P2Y
2
and P2Y
14
levels were highest, respectively. We extended these studies to investigate P2X
4
receptor mRNA in heart from rats with coronary artery ligation-induced heart failure. P2X
4
receptor mRNA was upregulated by 93% in SAN (
P
< 0.05), while a trend towards an increase was also observed in the right atrium and left ventricle (not significant). Thus, P2X
4
-mediated effects might be modulated in heart failure. mRNA for P2X
4–7
and P2Y
1,2,4,6,12–14
, but not P2X
2,3
and P2Y
11
, was detected in human right atrium and SAN. In addition, mRNA for P2X
1
was detected in human SAN but not human right atrium. In human right atrium and SAN, P2X
4
and P2X
7
mRNA was the highest for P2X receptors. P2Y
1
and P2Y
2
mRNA were the most abundant for P2Y receptors in the right atrium, while P2Y
1
, P2Y
2
, and P2Y
14
were the most abundant P2Y receptor subtypes in human SAN. This study shows a widespread distribution of P2 receptor mRNA in rat heart tissues but a more restricted presence and distribution of P2 receptor mRNA in human atrium and SAN. This study provides further direction for the elucidation of P2 receptor modulation of heart rate and contractility.</description><subject>Adult</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myocardium - chemistry</subject><subject>Myocardium - metabolism</subject><subject>Neurosciences</subject><subject>Original Aricle</subject><subject>Pharmacology/Toxicology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Purinergic P2 - analysis</subject><subject>Receptors, Purinergic P2 - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Sinoatrial Node - chemistry</subject><subject>Sinoatrial Node - metabolism</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kLtOwzAUhi0EoqXwACzIG1Pg2M7NY1VxkypAqMyWYzttqsQOdjLw9rikEhtn8fBfzvGH0DWBOwJQ3AcASiAB4AmkwBJ6guYkZTQhnNBTNI9ymRDKyxm6CGEPADnJsnM0I5yyNC_5HG3eKe5H31jjt43C3ijTD87j7uN1iRuLvRywtBrvxk5aHBrr5OAb2WLrtPlV3LAzHu-M9EOMbxtnwyU6q2UbzNXxXaDPx4fN6jlZvz29rJbrRKXAh4TmRVZpHW-STJdM0aoimuYpi6Og5opVuVQpKWqW6ZIWBQfFdUVSSbjmdckW6Hbq7b37Gk0YRNcEZdpWWuPGIIpYRIu4KzrJ5FTeheBNLXrfdNJ_CwLiwFJMLEVkKQ4sBY2Zm2P7WHVG_yWO8KKBToYQJbs1Xuzd6G388T-tPz3xffc</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Musa, Hanny</creator><creator>Tellez, James O.</creator><creator>Chandler, Natalie J.</creator><creator>Greener, Ian D.</creator><creator>Mączewski, Michał</creator><creator>Mackiewicz, Urszula</creator><creator>Beresewicz, Andrzej</creator><creator>Molenaar, Peter</creator><creator>Boyett, Mark R.</creator><creator>Dobrzynski, Halina</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions</title><author>Musa, Hanny ; Tellez, James O. ; Chandler, Natalie J. ; Greener, Ian D. ; Mączewski, Michał ; Mackiewicz, Urszula ; Beresewicz, Andrzej ; Molenaar, Peter ; Boyett, Mark R. ; Dobrzynski, Halina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myocardium - chemistry</topic><topic>Myocardium - metabolism</topic><topic>Neurosciences</topic><topic>Original Aricle</topic><topic>Pharmacology/Toxicology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Purinergic P2 - analysis</topic><topic>Receptors, Purinergic P2 - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Sinoatrial Node - chemistry</topic><topic>Sinoatrial Node - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Musa, Hanny</creatorcontrib><creatorcontrib>Tellez, James O.</creatorcontrib><creatorcontrib>Chandler, Natalie J.</creatorcontrib><creatorcontrib>Greener, Ian D.</creatorcontrib><creatorcontrib>Mączewski, Michał</creatorcontrib><creatorcontrib>Mackiewicz, Urszula</creatorcontrib><creatorcontrib>Beresewicz, Andrzej</creatorcontrib><creatorcontrib>Molenaar, Peter</creatorcontrib><creatorcontrib>Boyett, Mark R.</creatorcontrib><creatorcontrib>Dobrzynski, Halina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Musa, Hanny</au><au>Tellez, James O.</au><au>Chandler, Natalie J.</au><au>Greener, Ian D.</au><au>Mączewski, Michał</au><au>Mackiewicz, Urszula</au><au>Beresewicz, Andrzej</au><au>Molenaar, Peter</au><au>Boyett, Mark R.</au><au>Dobrzynski, Halina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions</atitle><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle><stitle>Naunyn-Schmied Arch Pharmacol</stitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>379</volume><issue>6</issue><spage>541</spage><epage>549</epage><pages>541-549</pages><issn>0028-1298</issn><eissn>1432-1912</eissn><abstract>It is known that adenosine 5′-triphosphate (ATP) is a cotransmitter in the heart. Additionally, ATP is released from ischemic and hypoxic myocytes. Therefore, cardiac-derived sources of ATP have the potential to modify cardiac function. ATP activates P2X
1–7
and P2Y
1–14
receptors; however, the presence of P2X and P2Y receptor subtypes in strategic cardiac locations such as the sinoatrial node has not been determined. An understanding of P2X and P2Y receptor localization would facilitate investigation of purine receptor function in the heart. Therefore, we used quantitative PCR and in situ hybridization to measure the expression of mRNA of all known purine receptors in rat left ventricle, right atrium and sinoatrial node (SAN), and human right atrium and SAN. Expression of mRNA for all the cloned P2 receptors was observed in the ventricles, atria, and SAN of the rat. However, their abundance varied in different regions of the heart. P2X
5
was the most abundant of the P2X receptors in all three regions of the rat heart. In rat left ventricle, P2Y
1
, P2Y
2
, and P2Y
14
mRNA levels were highest for P2Y receptors, while in right atrium and SAN, P2Y
2
and P2Y
14
levels were highest, respectively. We extended these studies to investigate P2X
4
receptor mRNA in heart from rats with coronary artery ligation-induced heart failure. P2X
4
receptor mRNA was upregulated by 93% in SAN (
P
< 0.05), while a trend towards an increase was also observed in the right atrium and left ventricle (not significant). Thus, P2X
4
-mediated effects might be modulated in heart failure. mRNA for P2X
4–7
and P2Y
1,2,4,6,12–14
, but not P2X
2,3
and P2Y
11
, was detected in human right atrium and SAN. In addition, mRNA for P2X
1
was detected in human SAN but not human right atrium. In human right atrium and SAN, P2X
4
and P2X
7
mRNA was the highest for P2X receptors. P2Y
1
and P2Y
2
mRNA were the most abundant for P2Y receptors in the right atrium, while P2Y
1
, P2Y
2
, and P2Y
14
were the most abundant P2Y receptor subtypes in human SAN. This study shows a widespread distribution of P2 receptor mRNA in rat heart tissues but a more restricted presence and distribution of P2 receptor mRNA in human atrium and SAN. This study provides further direction for the elucidation of P2 receptor modulation of heart rate and contractility.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19234689</pmid><doi>10.1007/s00210-009-0403-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-1298 |
| ispartof | Naunyn-Schmiedeberg's archives of pharmacology, 2009-06, Vol.379 (6), p.541-549 |
| issn | 0028-1298 1432-1912 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733327409 |
| source | Springer Nature |
| subjects | Adult Animals Biomedical and Life Sciences Biomedicine Female Humans Male Middle Aged Myocardium - chemistry Myocardium - metabolism Neurosciences Original Aricle Pharmacology/Toxicology Rats Rats, Wistar Receptors, Purinergic P2 - analysis Receptors, Purinergic P2 - metabolism RNA, Messenger - analysis RNA, Messenger - metabolism Sinoatrial Node - chemistry Sinoatrial Node - metabolism |
| title | P2 purinergic receptor mRNA in rat and human sinoatrial node and other heart regions |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T11%3A00%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=P2%20purinergic%20receptor%20mRNA%20in%20rat%20and%20human%20sinoatrial%20node%20and%20other%20heart%20regions&rft.jtitle=Naunyn-Schmiedeberg's%20archives%20of%20pharmacology&rft.au=Musa,%20Hanny&rft.date=2009-06-01&rft.volume=379&rft.issue=6&rft.spage=541&rft.epage=549&rft.pages=541-549&rft.issn=0028-1298&rft.eissn=1432-1912&rft_id=info:doi/10.1007/s00210-009-0403-2&rft_dat=%3Cproquest_cross%3E733327409%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c409t-2675bdd615a3d83c2bb1d2643333c0f9c3b6ac417f35d827790c9db14a19d9f83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733327409&rft_id=info:pmid/19234689&rfr_iscdi=true |