Influence of pressure on the retention of sugars in hydrophilic interaction chromatography

Pressure can influence the retention of analytes in hydrophilic interaction chromatography as well as in RP chromatography. We demonstrate that the retention of sugars in hydrophilic interaction chromatography decreases with pressure, and interpret the observation as a gain in solvation, or more spe...

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Bibliographic Details
Published in:Journal of separation science 2010-03, Vol.33 (6-7), p.838-840
Main Authors: Neue, Uwe D, Hudalla, Chris J, Iraneta, Pamela C
Format: Article
Language:English
Subjects:
Chromatography
Gain
HILIC
Hydration
Pressure
Separation
Solvation
Sugars
UPLC
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Summary:Pressure can influence the retention of analytes in hydrophilic interaction chromatography as well as in RP chromatography. We demonstrate that the retention of sugars in hydrophilic interaction chromatography decreases with pressure, and interpret the observation as a gain in solvation, or more specifically hydration, as the sugar molecules enter the water-rich stationary phase.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900628