Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts

• Published in: The Journal of nutritional biochemistry 2010-07, Vol.21 (7), p.659-664
• Main Authors: Hosokawa, Yoshitaka, Hosokawa, Ikuko, Ozaki, Kazumi, Nakanishi, Tadashi, Nakae, Hideaki, Matsuo, Takashi
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Antioxidants - pharmacology; Biological and medical sciences; Catechin - analogs & derivatives; Catechin - pharmacology; Catechins; Cells, Cultured; Chemokine CXCL10 - metabolism; CXCL10; Cytokine Receptor gp130 - genetics; Cytokine Receptor gp130 - metabolism; Enzyme Inhibitors - pharmacology; Feeding. Feeding behavior; Fibroblasts - drug effects; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gingiva - cytology; Gingiva - drug effects; Gingiva - metabolism; Human gingival fibroblasts; Humans; Oncostatin M; Oncostatin M - pharmacology; Oncostatin M Receptor beta Subunit - genetics; Oncostatin M Receptor beta Subunit - metabolism; Osmolar Concentration; Periodontal Diseases - prevention & control; Phosphorylation - drug effects; Signal Transduction - drug effects; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2009.04.005

CXC chemokine ligand 10 (CXCL10) plays a pivotal role in the recruitment of Th1 cells and, thus, in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins derived from green tea, have multiple beneficial effects, but the effects of catechins on CXCL10 production from human gingival fibroblasts (HGFs) is not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit oncostatin M (OSM)-induced CXCL10 production in HGFs. HGFs constitutively expressed glycoprotein 130 and OSM receptor beta (OSMRβ), which are OSM receptors. OSM increased CXCL10 production in a concentration-dependent manner. EGCG and ECG prevented OSM-mediated CXCL10 production by HGFs. Inhibitors of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase (JNK), phosphatidylinositol-3-OH kinase and signal transducer and activator of transcription (STAT)3 decreased OSM-induced CXCL10 production. EGCG significantly prevented OSM-induced phosphorylation of JNK, Akt (Ser473) and STAT3 (Tyr705 and Ser727). ECG prevented phosphorylation of JNK and Akt (Ser473). In addition, EGCG and ECG attenuated OSMRβ expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids, catechins, can provide direct benefits in periodontal disease.