Apoptosis as a Mechanism for the Elimination of Cardiomyocytes After Acute Myocardial Infarction

Apoptosis is recognized as a mechanism of cell loss in the setting of acute myocardial infarction (AMI). Whether it contributes to myocyte elimination late after AMI has not yet been confirmed. We attempted to identify the features of apoptosis in myocytes that survived AMI. A search for ongoing apo...

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Bibliographic Details
Published in:The American journal of cardiology 2010-05, Vol.105 (9), p.1240-1245
Main Authors: Prech, Marek, MD, Marszałek, Andrzej, MD, PhD, Schröder, Josef, PhD, MSc, Filas, Violetta, MSc, Lesiak, Maciej, MD, PhD, Jemielity, Marek, MD, PhD, Araszkiewicz, Aleksander, MD, Grajek, Stefan, MD, PhD
Format: Article
Language:English
Subjects:
Adult
Aged
Apoptosis
Biological and medical sciences
Cadaver
Cardiology
Cardiology. Vascular system
Cardiovascular
Caspase 3 - metabolism
Cell growth
Cells
Coronary heart disease
Heart
Heart attacks
Humans
Immunohistochemistry
Medical sciences
Microscopy, Electron, Transmission
Middle Aged
Myocardial Infarction - metabolism
Myocardial Infarction - pathology
Myocarditis. Cardiomyopathies
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - ultrastructure
Proto-Oncogene Proteins c-bcl-2 - metabolism
Transmission electron microscopy
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Summary:Apoptosis is recognized as a mechanism of cell loss in the setting of acute myocardial infarction (AMI). Whether it contributes to myocyte elimination late after AMI has not yet been confirmed. We attempted to identify the features of apoptosis in myocytes that survived AMI. A search for ongoing apoptosis was performed in samples obtained from 38 human hearts: group I (n = 10), noncardiac death (control); and group II (n = 28), left ventricular aneurysm (in 20 patients, the samples were collected during aneurysmectomy and from 8 at autopsy). The morphometric evaluation included the degree of cellular hypertrophy, density of the capillary network, extent of myocytolysis, and features of apoptosis. Immunohistochemistry for caspase-3 and Bcl-2 was used as a prerequisite for transmission electron microscopy. Slides showing the strongest reaction for caspase-3 and negative for Bcl-2 were selected for transmission electron microscopy. CD-34 immunohistochemistry was used to quantify the capillary density. A significant reduction in capillary density was observed compared to the control group (1,085.6 ± 205.0/mm2 vs 2,968.7 ± 457.3/mm2 ; p
ISSN:0002-9149
1879-1913
DOI:10.1016/j.amjcard.2009.12.039