Intestinal Microbial Ecology in Premature Infants Assessed with Non–Culture-Based Techniques

Objectives To use high throughput techniques to analyze intestinal microbial ecology in premature neonates, who are highly susceptible to perturbations of the luminal environment associated with necrotizing enterocolitis (NEC) and late-onset sepsis. Study design With non–culture-based techniques, we...

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Bibliographic Details
Published in:The Journal of pediatrics 2010, Vol.156 (1), p.20-25
Main Authors: Mshvildadze, Maka, MD, Neu, Josef, MD, Shuster, Jonathan, PhD, Theriaque, Douglas, MS, Li, Nan, MS, MD, Mai, Volker, MPH, PhD
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA, Bacterial - analysis
Enterocolitis, Necrotizing - microbiology
Feces - microbiology
Female
General aspects
Humans
Infant, Newborn
Infant, Premature
Infant, Premature, Diseases - microbiology
Klebsiella
Male
Meconium - microbiology
Medical sciences
Pediatrics
Polymerase Chain Reaction - methods
RNA, Ribosomal, 16S
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Summary:Objectives To use high throughput techniques to analyze intestinal microbial ecology in premature neonates, who are highly susceptible to perturbations of the luminal environment associated with necrotizing enterocolitis (NEC) and late-onset sepsis. Study design With non–culture-based techniques, we evaluated intestinal microbiota shortly after birth and during hospitalization in 23 neonates born at 23 to 32 weeks gestational age. Microbiota compositions were compared in 6 preterm infants in whom NEC, signs of systemic inflammation, or both developed with matched control subjects by using 16S ribosomal RNA pyrosequencing. Results Microbial DNA was detected in meconium, suggesting an intrauterine origin. Differences in diversity were detected in infants whose mothers intended to breast feed ( P = .03), babies born to mothers with chorioamnionitis ( P  = .06), and in babies born at
ISSN:0022-3476
1097-6833
DOI:10.1016/j.jpeds.2009.06.063