Clinical and serological evaluation of Leptospirosis in Puducherry, India

Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by p...

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Bibliographic Details
Published in:Journal of infection in developing countries 2010-03, Vol.4 (3), p.139-143
Main Authors: Shekatkar, Smita Benjamin, Harish, Belgode Narasimha, Menezes, Godfred Antony, Parija, Subhash Chandra
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Agglutination Tests - methods
Antibodies, Bacterial - blood
Diagnostic Techniques and Procedures
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Immunoglobulin M - blood
India - epidemiology
Leptospira - immunology
Leptospirosis
Leptospirosis - blood
Leptospirosis - diagnosis
Leptospirosis - epidemiology
Leptospirosis - immunology
Male
Middle Aged
Prevalence
Sensitivity and Specificity
Seroepidemiologic Studies
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Summary:Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by performing Microscopic Agglutination Test (MAT). The other aim of this study was to compare the results of a commercially available IgM ELISA with that of MAT. A total of 110 blood samples from patients suspected of leptospirosis were sent for diagnosis. These samples were subjected to IgM ELISA and the microscopic agglutination test (MAT). MAT was done using a panel of 12 Leptospira serovars. MAT analysis of the 110 samples showed 40 (36%) to be positive. Antibodies were predominantly seen against serogroup Leptospira Icterohemorrhagiae (27%), followed by Pomona (17%), and Pyrogenes (12%). IgM ELISA done on these samples showed a positivity of 37% compared to MAT. This study reveals that the MAT test can be standardized in a diagnostic laboratory and used in conjunction with an IgM ELISA.
ISSN:1972-2680
2036-6590
1972-2680
DOI:10.3855/jidc.384