Absence of the BRAF Mutation in HBME1 + and CK19+ Atypical Cell Clusters in Hashimoto Thyroiditis: Supportive Evidence Against Preneoplastic Change

An association between Hashimoto thyroiditis and papillary thyroid carcinoma has been postulated for decades. We undertook this study to identify potential precursors of papillary thyroid carcinoma in Hashimoto thyroiditis using a combination of morphologic, immunohistochemical, and molecular techni...

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Bibliographic Details
Published in:American journal of clinical pathology 2009-12, Vol.132 (6), p.906-912
Main Authors: NASR, Michel R, MUKHOPADHYAY, Sanjay, SHENGLE ZHANG, KATZENSTEIN, Anna-Luise A
Format: Article
Language:English
Subjects:
Adenocarcinoma, Papillary - genetics
Adenocarcinoma, Papillary - metabolism
Adenocarcinoma, Papillary - pathology
Biological and medical sciences
Biomarkers, Tumor - metabolism
Diagnosis, Differential
DNA Mutational Analysis
DNA, Neoplasm - analysis
Endocrinopathies
Hashimoto Disease - genetics
Hashimoto Disease - metabolism
Hashimoto Disease - pathology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Keratin-19 - metabolism
Medical sciences
Mutation
Non tumoral diseases. Target tissue resistance. Benign neoplasms
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Precancerous Conditions - genetics
Precancerous Conditions - metabolism
Precancerous Conditions - pathology
Proto-Oncogene Proteins B-raf - genetics
Proto-Oncogene Proteins B-raf - metabolism
Thyroid Neoplasms - genetics
Thyroid Neoplasms - metabolism
Thyroid Neoplasms - pathology
Thyroid. Thyroid axis (diseases)
Citations: Items that this one cites
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Summary:An association between Hashimoto thyroiditis and papillary thyroid carcinoma has been postulated for decades. We undertook this study to identify potential precursors of papillary thyroid carcinoma in Hashimoto thyroiditis using a combination of morphologic, immunohistochemical, and molecular techniques. For the study, samples from 59 cases of Hashimoto thyroiditis were stained with antibodies to HBME1 and cytokeratin (CK)19. Tiny HBME1+ and CK19+ atypical cell clusters were identified and analyzed for the BRAF mutation by the colorimetric Mutector assay and allele-specific polymerase chain reaction. HBME1+ and CK19+ atypical cell clusters were identified in 12 (20%) of 59 cases. The minute size (
ISSN:0002-9173
1943-7722
DOI:10.1309/AJCPCGCZZ1OYF0IC