Effect of intra-ischemic hypothermia on the expression of c-Fos and c-Jun, and DNA binding activity of AP-1 after focal cerebral ischemia in rat brain

• Published in: Brain research 2003-06, Vol.975 (1), p.149-157
• Main Authors: Akaji, Kazunori, Suga, Sadao, Fujino, Tadahiro, Mayanagi, Keita, Inamasu, Joji, Horiguchi, Takashi, Sato, Shuzo, Kawase, Takeshi
• Format: Article
• Language: English
• Subjects: Animals; AP-1; Biological and medical sciences; Body Temperature - physiology; Brain Chemistry - physiology; Brain Ischemia - metabolism; c-Fos; c-Jun; Cerebral Infarction - pathology; DNA - metabolism; Electrophoretic Mobility Shift Assay; Focal cerebral ischemia; Hypothermia; Hypothermia - metabolism; Immunohistochemistry; Male; Medical sciences; Neurology; Proto-Oncogene Proteins c-fos - biosynthesis; Proto-Oncogene Proteins c-jun - biosynthesis; Rats; Rats, Wistar; Transcription Factor AP-1 - metabolism; Vascular diseases and vascular malformations of the nervous system
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(03)02622-2

It is unknown whether immediate early gene (IEG) induction and subsequent late gene regulation after ischemia is beneficial or deleterious. The aim of this study was to examine the effect of hypothermia on expression of c-Fos and c-Jun, and AP-1 DNA binding activity, after transient focal cerebral ischemia in rat brain, and clarify the role of IEGs and AP-1 after insults. Male Wistar rats underwent right middle cerebral artery occlusion for 1 h with the intraluminal suture method. During ischemia, animals were assigned to either normothermic (NT) or hypothermic (HT) groups. In the NT group, brain temperature was observed to spontaneously increase to 40 °C during ischemia. In the HT group, brain temperature decreased to 30 °C. Infarct volume in cortex was decreased in the HT group, compared with that in the NT group ( P