Simultaneous detection of N-acetyl-l-cysteine and physiological low molecular mass thiols in plasma by capillary electrophoresis

N-acetyl-l-cysteine (NAC) is a therapeutic drug widely used as mucolytic agent in the treatment of respiratory diseases. Recently it has been proposed that NAC administration may modify the plasma levels of low molecular weight thiols (LMW) like cysteine, homocysteine and glutathione, though it has...

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Bibliographic Details
Published in:Amino acids 2009-07, Vol.37 (2), p.395-400
Main Authors: Zinellu, Angelo, Sotgia, Salvatore, Scanu, Bastianina, Usai, Maria Franca, Fois, Alessandro Giuseppe, Spada, Valentina, Deledda, Anna, Deiana, Luca, Pirina, Piero, Carru, Ciriaco
Format: Article
Language:English
Subjects:
Acetylcysteine - blood
Acetylcysteine - therapeutic use
Amino acids
Analytical Chemistry
Biochemical Engineering
Biochemistry
Biomedical and Life Sciences
Capillarity
Cysteine
Drugs
Electrophoresis
Electrophoresis, Capillary - methods
Expectorants - metabolism
Expectorants - therapeutic use
Homocysteine
Humans
Life Sciences
Low molecular weights
Molecular Weight
Neurobiology
Original Article
Plasma
Proteomics
Pulmonary Disease, Chronic Obstructive - blood
Respiratory diseases
Sulfhydryl Compounds - blood
Sulfhydryl Compounds - chemistry
Thiols
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Summary:N-acetyl-l-cysteine (NAC) is a therapeutic drug widely used as mucolytic agent in the treatment of respiratory diseases. Recently it has been proposed that NAC administration may modify the plasma levels of low molecular weight thiols (LMW) like cysteine, homocysteine and glutathione, though it has been still debated if their plasma concentration increases or decreases during the therapy. Therefore research calls for methods able to analyze simultaneously NAC and the other plasma LMW thiols in order to evaluate if NAC is able to modify plasma thiols concentration and in particular to reduce homocysteine levels in hyperhomocysteinemia. In this paper we present a new capillary electrophoresis method that allows a baseline separation of plasma NAC from the physiological thiols. The proposed method has been utilized to measure the drug and the physiological LMW thiols in NAC administered chronic obstructive broncho-pneumopathy (COPB) disease patients.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-008-0167-x