Cgr11 encodes a secretory protein involved in cell adhesion

We performed comparative proteomic analyses of pituitary tumor-derived cell lines, and found a new protein, preliminarily called hydrophobestin, which was produced only in somatotrophic cells, MtT/S, but not in non-hormone-producing cells, MtT/E. Hydrophobestin is encoded by the cell growth regulato...

Full description

Saved in:
Bibliographic Details
Published in:European journal of cell biology 2009-09, Vol.88 (9), p.521-529
Main Authors: Devnath, Sukumar, Kataoka, Taro, Miura, Kaoru, Kusuda, Mie, Kitamura, Kunio, Kumada, Yuko, Mochiduki, Akikazu, Kaneko, Kei, Adachi, Akihito, Inoue, Kinji
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - metabolism
Cell Line
Cgr11
EF-hand motif
Fluorescent Antibody Technique
Gene Expression
Immunohistochemistry
PC12 Cells
Rats
Secretory protein
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We performed comparative proteomic analyses of pituitary tumor-derived cell lines, and found a new protein, preliminarily called hydrophobestin, which was produced only in somatotrophic cells, MtT/S, but not in non-hormone-producing cells, MtT/E. Hydrophobestin is encoded by the cell growth regulatory gene, Cgr11, which is known to have growth-suppressive potential in several cell lines. We have now sought to investigate the underlying events responsible for cell growth inhibition by hydrophobestin. Immunocytochemisty revealed that hydrophobestin is localized in the Golgi apparatus of MtT/S cells and Cgr11-transfected MtT/E cells. The apparent molecular mass of the protein was determined by Westerm blot analysis of conditioned culture medium of MtT/S cells. Our data show that hydrophobestin is a secretory protein localized in the pituitary gland, adrenal gland, digestive tract, reproductive organs, and kidney. We also found that hydrophobestin promotes compact monolayer cell aggregates in PC12 cells transfected with Cgr11, however, non-transfected, vector- or EF-hand motif-deleted (ΔEF) Cgr11-transfected PC12 cells cannot form compact cell colonies. An antibody recognizing EF-hand motifs showed strong staining in the intercellular space of both Cgr11-transfected PC12 cells and MtT/S cells ( Cgr11-expressing cells). Our data suggest that hydrophobestin-mediated cell adhesion may regulate cell growth through compact cell attachment.
ISSN:0171-9335
1618-1298
DOI:10.1016/j.ejcb.2009.04.003