Association of the MCP-1 -2518 A/G Polymorphism and No Association of Its Receptor CCR2 -64 V/I Polymorphism with Lupus Nephritis

• Published in: Journal of rheumatology 2010-04, Vol.37 (4), p.776-782
• Main Authors: MALAFRONTE, Patricia, VIEIRA, Jose Mauro, PEREIRA, Alexandre Carlos, KRIEGER, Jose Eduardo, BARROS, Rui Toledo, WORONIK, Viktoria
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Analysis of Variance; Biological and medical sciences; Chemokine CCL2 - genetics; Creatinine - blood; Diseases of the osteoarticular system; Female; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Kidney - physiopathology; Kidney Function Tests; Kidneys; Lupus Nephritis - blood; Lupus Nephritis - genetics; Lupus Nephritis - physiopathology; Male; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; Patient Selection; Polymerase Chain Reaction; Polymorphism, Single Nucleotide - genetics; Receptors, CCR2 - genetics; Severity of Illness Index; Statistics, Nonparametric; Urinary system involvement in other diseases. Miscellaneous
• ISSN: 0315-162X; 1499-2752
• DOI: 10.3899/jrheum.090681

To evaluate whether the A/G polymorphism at position -2518 in the regulatory region of the monocyte chemoattractant protein-1 (MCP-1) or the V/I polymorphism at position -64 of the receptor, CCR2, are associated with lupus nephritis (LN) or any clinical characteristics of the disease or with renal survival in a patient population. We selected 197 patients with lupus nephritis and 220 matched healthy controls for study. MCP-1 and CCR2 genotyping was performed by polymerase chain reaction. Clinical and laboratory data were compiled from patients' charts over followup that ranged from 6 months to 10 years. The G/G genotype of MCP-1 was more common in LN patients (p = 0.019), while the A allele was associated with healthy controls (p = 0.007) as was the V allele of CCR2 (p = 0.046) compared to LN patients. Clinical index measures [SLE Disease Activity Index (SLEDAI)], immunological markers, renal histology, renal function at enrollment, and renal survival were not influenced by these polymorphisms. A less aggressive renal disease, measured by renal SLEDAI index, was associated with the V allele of the CCR2 gene polymorphism. These findings support that MCP-1 -2518 G/G is associated with LN but there was no association of this genotype with renal function or renal survival. When studying CCR2 -64 V/I polymorphism we showed a positive association of the V allele with healthy controls but no association of the genotype with LN patients.