Resistance of Enterobacter sakazakii (Cronobacter spp.) to environmental stresses

• Published in: Journal of applied microbiology 2009-11, Vol.107 (5), p.1606-1614
• Main Authors: Dancer, G.I, Mah, J.-H, Rhee, M.-S, Hwang, I.-G, Kang, D.-H
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Biological and medical sciences; Colony Count, Microbial; Cronobacter sakazakii; Cronobacter sakazakii - growth & development; Cronobacter sakazakii - physiology; Desiccation; dry stress; Enterobacter sakazakii; Fundamental and applied biological sciences. Psychology; Hot Temperature; Hydrogen-Ion Concentration; Microbiology; thermal stress; Time Factors; Water; water activity
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2009.04347.x

To gain a better understanding of the survival and persistence of Enterobacter sakazakii in severe environments. We evaluated the resistance of Ent. sakazakii to various environmental stresses, including heating, drying, water activity (aw), and pH. The resistance of Ent. sakazakii to heat varies widely among strains. Most tested strains of Ent. sakazakii exhibited unusual resistance to dry stress, which depends on drying media. Growth of most strains occurred within 24 h at 37°C when the initial aw of the medium was adjusted to 0·94 with sucrose or sodium chloride. The minimum pH for growth within 24 h at 37°C was 3·9 or 4·1 for most strains tested. Additionally, there did not appear to be any relationship between resistance to stresses and biofilm-forming ability in Ent. sakazakii planktonic cells. These results indicate that Ent. sakazakii is much more resistant than other Enterobacteriaceae to environmental stresses. Moreover, it is likely that Ent. sakazakii has cross-resistance to dry and thermal stresses. The findings of this study will contribute to an improved understanding of the survival and behaviour of Ent. sakazakii, which will lead to improved strategies for preventing outbreaks of Ent. sakazakii infection.