2-layer based microfluidic concentration generator by hybrid serial and volumetric dilutions

We present a 2-layer based microfluidic concentration generator by a hybrid of a serial and a volumetric dilution for dose-response experiments in drug screening. The hybrid dilution method using 2-layer based microfluidic network significantly reduces the total number of cascaded serial dilution st...

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Bibliographic Details
Published in:Biomedical microdevices 2010-04, Vol.12 (2), p.297-309
Main Authors: Lee, Kangsun, Kim, Choong, Kim, Youngeun, Jung, Keunhui, Ahn, Byungwook, Kang, Ji Yoon, Oh, Kwang W
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biophysics
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical - instrumentation
Engineering
Engineering Fluid Dynamics
Fluids
Humans
Hybridization
Indicator Dilution Techniques - instrumentation
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics - instrumentation
Microfluidics - methods
Nanotechnology
Software
Volumetric analysis
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Summary:We present a 2-layer based microfluidic concentration generator by a hybrid of a serial and a volumetric dilution for dose-response experiments in drug screening. The hybrid dilution method using 2-layer based microfluidic network significantly reduces the total number of cascaded serial dilution stages. The proposed strategy is capable of generating a large number of universal stepwise monotonic concentrations with a wide range of logarithmic and linear scales. We have studied an equivalent electrical circuit to that of the 2-layer based microfluidic network, where the only variable parameter is channel length. We have designed a microfluidic dilution generator simultaneously covering 14 doses with a combination of 4-order logarithmic and 4-point linear concentrations. The design has been verified by a commercial circuit analysis software (e.g., P-Spice) for the electrical circuit analysis and a computational fluid dynamics software (e.g., CFD-ACE⁺) for the microfluidic circuit analysis. As a real-life application of the proposed dilution generator, we have successfully performed a dose-response experiment using MCF-7 human breast cancer cells. We expect that the proposed dilution method will be useful to study not only high throughput drug screening but also optimization in biology, chemistry, medicine, and material sciences.
ISSN:1387-2176
1572-8781
DOI:10.1007/s10544-009-9385-6