Spatial Correlation of Confocal Raman Scattering and Secondary Ion Mass Spectrometric Molecular Images of Lignocellulosic Materials

A detailed chemical and structural understanding of pre-enzymatic processing of lignocellulosic materials (LCMs) is a key objective in the development of renewable energy. Efficient rendering of biomass components into fermentable substrates for conversion into biofuel feedstocks would benefit great...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2010-04, Vol.82 (7), p.2608-2611
Main Authors: Li, Zhen, Chu, Li-Qiang, Sweedler, Jonathan V, Bohn, Paul W
Format: Article
Language:English
Subjects:
Alternative energy
Analytical chemistry
Biomass
Chemistry
Enzymes
Exact sciences and technology
Ions - chemistry
Lignin - chemistry
Mass spectrometry
Poaceae - chemistry
Polysaccharides - chemistry
Scattering
Spectrometric and optical methods
Spectrometry, Mass, Electrospray Ionization - methods
Spectrum Analysis, Raman - methods
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Summary:A detailed chemical and structural understanding of pre-enzymatic processing of lignocellulosic materials (LCMs) is a key objective in the development of renewable energy. Efficient rendering of biomass components into fermentable substrates for conversion into biofuel feedstocks would benefit greatly from the development of new technologies to provide high-quality, spatially resolved chemical information about LCMs during the various processing states. In an effort to realize this important goal, spatially correlated confocal Raman and mass spectrometric images allow the extraction of three-dimensional information from the perennial grass, Miscanthus x giganteus. An optical microscopy-based landmark registry scheme was developed that allows samples to be transferred between laboratories at different institutions, while retaining the capability to access the same physical regions of the samples. Subsequent to higher resolution imaging via confocal Raman microscopy and secondary ion mass spectrometry (SIMS), laser desorption-ionization mass spectrometry was used to place these regions within the overall sample architecture. Excellent sample registry was evident in the highly correlated Raman and SIMS images. In addition, the correlation of vibrational Raman scattering with mass spectra from specific spatial locations allowed confirmation of the assignment of intracellular globular structures to hemicellulose-rich lignin complexes, an assignment which could only be made tentatively from either image alone.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac100026r