Multiple ovarian transplants to rescue a transgenic line of mice

Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovarie...

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Bibliographic Details
Published in:Lab animal 2010-06, Vol.39 (6), p.191-193
Main Authors: Dawes, Joyce, Liu, Bowen, Mars, Wendy, Michalopoulos, George, Khillan, Jaspal S
Format: Article
Language:English
Subjects:
Animal experimentation
Animal Models
Animals
Biomedical and Life Sciences
Breeding - methods
breeding lines
Cryopreservation - veterinary
Female
Fertility - physiology
Genetic engineering
Genetically modified animals
Health aspects
laboratory animals
Life Sciences
Methods
Mice
Mice, Transgenic
organ transplantation
Organ Transplantation - veterinary
ovarian transplants
Ovaries
Ovary - surgery
Ovary - transplantation
Reproductive Techniques, Assisted - veterinary
technique
Tissue and Organ Procurement - methods
transgenic animals
Transplantation
Transplantation of organs, tissues, etc
Veterinary Medicine/Veterinary Science
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Summary:Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovaries from a donor transgenic mouse, cryopreserve the ovarian tissue, transplant this tissue into histocompatible female mice and breed these recipient females. Though it is a useful technique, cryopreservation can potentially damage ovarian tissue, which could reduce fertility. In this article, the authors describe how they carried out ovarian transplants without cryopreservation to rescue a line of transgenic C57BL/6 mice. Other researchers who have experience with mouse reproductive surgery should be able to use this technique to rescue infertile transgenic lines of mice.
ISSN:0093-7355
1548-4475
DOI:10.1038/laban0610-191