HRP-based biosensor for monitoring rifampicin

• Published in: Biosensors & bioelectronics 2003-08, Vol.18 (9), p.1165-1171
• Main Authors: Alonso Lomillo, M.A., Kauffmann, J.M., Arcos Martinez, M.J.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Coated Materials, Biocompatible - chemistry; Drug Contamination - prevention & control; Electrochemistry - instrumentation; Electrochemistry - methods; Electrodeposited polypyrrole; Enzymes, Immobilized - chemistry; Experimental design; Fundamental and applied biological sciences. Psychology; Horseradish Peroxidase - chemistry; HRP biosensor; Methods. Procedures. Technologies; Polymers - chemistry; Pyrroles - chemistry; Reproducibility of Results; Rifampicin; Rifampin - analysis; Rifampin - chemistry; Sensitivity and Specificity; Urinalysis - instrumentation; Urinalysis - methods; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(02)00251-8

Pyrrole was electropolymerized onto a Pt electrode in the presence of LiClO 4 and horseradish peroxidase (HRP). This HRP-based biosensor has been used for the amperometric detection of rifampicin (RIF) in the presence of a constant concentration of H 2O 2. The C H 2O 2 as well as the applied potential ( E ap) and the pH of the phosphate buffer have simultaneously been optimized through a central composite design. Under these conditions, repeatability, reproducibility, and stability of the modified electrode have been analyzed. The detection limit for RIF has been calculated taking into account the probability of false-positive ( α) and -negative ( β), reaching a value of 5.06×10 −6 mol dm −3. The biosensor was applied to the determination of RIF in pharmaceutical preparations and biological samples.