Direct Determination of Benzamides in Serum by Column-Switching High-Performance Liquid Chromatography

A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was...

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Bibliographic Details
Published in:Analytical Sciences 2003, Vol.19(5), pp.785-789
Main Authors: CHIBA, Ryoko, OGASAWARA, Ayako, KUBO, Teppei, YAMAZAKI, Hiroyuki, UMINO, Masuo, ISHIZUKA, Yoichi
Format: Article
Language:English
Subjects:
Analysis
Benzamides - blood
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid - methods
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Spectrometry, Fluorescence - methods
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Summary:A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.19.785