novel basic fibroblast growth factor delivery system fabricated with heparin-incorporated fibrin-fibronectin matrices for repairing rat sciatic nerve disruptions

Autologous nerve grafts are widely used in bridging critical gaps of peripheral nerves, but they remain associated with high morbidity of the donor site and lack of full recovery. As an alternative, we have focused on chitosan nerve conduits filled with a heparin-incorporated fibrin-fibronectin matr...

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Bibliographic Details
Published in:Biotechnology letters 2010-04, Vol.32 (4), p.585-591
Main Authors: Han, Hongyan, Ao, Qiang, Chen, Guoqiang, Wang, Shijie, Zuo, Huancong
Format: Article
Language:English
Subjects:
Action Potentials - drug effects
Animals
Applied Microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Chitosan - chemistry
Drug Delivery Systems - methods
Electrophysiology
Fibrin - administration & dosage
Fibrin - chemistry
Fibroblast Growth Factor 2 - administration & dosage
Fibronectins - administration & dosage
Fibronectins - chemistry
Fundamental and applied biological sciences. Psychology
Heparin - administration & dosage
Heparin - chemistry
Life Sciences
Male
Microbiology
Nerve Regeneration - drug effects
Neurological disorders
Neurology
Original Research Paper
Rats
Rats, Sprague-Dawley
Rodents
S100 Proteins - metabolism
Sciatic Nerve - anatomy & histology
Sciatic Nerve - drug effects
Sciatic Nerve - injuries
Sciatic Nerve - pathology
Sciatic Nerve - physiology
Tubulin - metabolism
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Summary:Autologous nerve grafts are widely used in bridging critical gaps of peripheral nerves, but they remain associated with high morbidity of the donor site and lack of full recovery. As an alternative, we have focused on chitosan nerve conduits filled with a heparin-incorporated fibrin-fibronectin matrix serving as delivery systems for basic fibroblast growth factor (bFGF). The artificial nerve conduits were used for repairing sciatic nerve defects of 10 mm in adult rats. Three months post-operation, the conduction velocity recovery index (CVRI) and the muscle restoration rate (MRR) in animals of the experimental group were 32 ± 4.1 and 77.4 ± 7.9%, respectively, which were significantly higher than those of the PBS control group (17.8 ± 1.9 and 66.7 ± 6.5%), and similar to those of the autograft group (38.4 ± 3.9 and 81.3 ± 7.8%). These results were also consistent with the densities of regenerated axons in the three groups, which were demonstrated by histomorphological analysis.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-009-0186-z