Quantitation of Chemical Warfare Agents Using the Direct Analysis in Real Time (DART) Technique

• Published in: Analytical chemistry (Washington) 2009-08, Vol.81 (16), p.6744-6749
• Main Authors: Nilles, J. Michael, Connell, Theresa R, Durst, H. Dupont
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Calibration; Chemical Warfare Agents - chemistry; Chemistry; Exact sciences and technology; General, instrumentation; Mass Spectrometry; Reproducibility of Results; Spectrometric and optical methods
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac900682f

Direct analysis in real time (DART) is an ion source that permits rapid mass spectrometric detection of gases, liquids, and solids in open air under ambient conditions. It is a unique technology in the field of chemical weapons detectors in that it does not require a vapor pressure, does not require sample preparation, and is nondestructive to the original sample. While the DART technique has had success as a first line instrument of detection, there have been lingering doubts over the technique’s quantitative reliability and reproducibility. Here, we demonstrate its capability to produce linear calibration curves (R 2 = 0.99 or better) for the nerve agents GA, GB, and VX as well as the blister agent HD. Independently prepared check standards measured against these curves typically have recovery errors less than 3%. We show the DART instrument response to be linear over roughly 3 orders of magnitude. Furthermore, this study shows that averaging as few as three measurements for each data point is sufficient to produce high quality calibration curves, thus reducing data collection time and providing quicker results.