An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells

• Published in: Plant molecular biology 1992-12, Vol.20 (5), p.821-831
• Main Authors: John, I. (Iowa State Univ., Ames, IA (USA). Dept. of Botany), Wang, H, Held, B.M, Syrkin Wurtele, E, Colbert, J.T
• Format: Article
• Language: English
• Subjects: ACIDE AMINE; ADN; Amino Acid Sequence; AMINO ACIDS; AMINOACIDOS; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; Cloning, Molecular; CORTEX; Cysteine - analysis; DNA; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Library; Genes; In Situ Hybridization; Leucine - analysis; Light-Harvesting Protein Complexes; MESSENGER RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Photosynthetic Reaction Center Complex Proteins - genetics; Plant Proteins - genetics; Proline - analysis; Protein Conformation; RACINE; RAICES; RNA, Messenger - genetics; RNA, Messenger - metabolism; ROOTS; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; ZEA MAYS; Zea mays - genetics; Zea mays - metabolism
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/bf00027153

A near full-length cDNA clone (pZRP3) corresponding to an mRNA that accumulates specifically in roots of maize was isolated. The ZRP3 mRNA is ca. 600 nucleotides in length. The amino acid sequence of the predicted polypeptide is rich in leucine (16%), proline (11%), and cysteine (8.5%). The zrp3 gene appears to be expressed exclusively in roots, whereas other ZRP3-related genes are expressed in additional organs of the maize plant. In situ hybridization shows that ZRP3 mRNA accumulation is largely confined to the cells of the cortical ground meristem. Furthermore, accumulation of this mRNA occurs within a distinct subset of cortical cells, the inner three to four cell layers.