Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail oval...

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Bibliographic Details
Published in:Folia microbiologica 1992, Vol.37 (4), p.273-278
Main Authors: KRIZKOVA, L, JELOKOVA, J, KLAUDINY, J, ZAMOCKY, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Coturnix
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression - genetics
Genetic engineering
Genetic technics
Genetic Vectors
Methods. Procedures. Technologies
Ovalbumin - biosynthesis
Ovalbumin - genetics
Plasmids
Recombinant Proteins - biosynthesis
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02814562