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Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae
A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail oval...
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Published in: | Folia microbiologica 1992, Vol.37 (4), p.273-278 |
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container_issue | 4 |
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container_title | Folia microbiologica |
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creator | KRIZKOVA, L JELOKOVA, J KLAUDINY, J ZAMOCKY, M |
description | A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection. |
doi_str_mv | 10.1007/BF02814562 |
format | article |
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The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/BF02814562</identifier><identifier>PMID: 1452097</identifier><identifier>CODEN: FOMIAZ</identifier><language>eng</language><publisher>Praha: Academia</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Coturnix ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Methods. Procedures. Technologies ; Ovalbumin - biosynthesis ; Ovalbumin - genetics ; Plasmids ; Recombinant Proteins - biosynthesis ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Vectors (cloning, transfer, expression). 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Psychology</topic><topic>Gene Expression - genetics</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Methods. Procedures. Technologies</topic><topic>Ovalbumin - biosynthesis</topic><topic>Ovalbumin - genetics</topic><topic>Plasmids</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KRIZKOVA, L</creatorcontrib><creatorcontrib>JELOKOVA, J</creatorcontrib><creatorcontrib>KLAUDINY, J</creatorcontrib><creatorcontrib>ZAMOCKY, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KRIZKOVA, L</au><au>JELOKOVA, J</au><au>KLAUDINY, J</au><au>ZAMOCKY, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>1992</date><risdate>1992</risdate><volume>37</volume><issue>4</issue><spage>273</spage><epage>278</epage><pages>273-278</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><coden>FOMIAZ</coden><abstract>A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. 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ispartof | Folia microbiologica, 1992, Vol.37 (4), p.273-278 |
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source | Springer LINK Archives |
subjects | Animals Biological and medical sciences Biotechnology Coturnix DNA - genetics Fundamental and applied biological sciences. Psychology Gene Expression - genetics Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Ovalbumin - biosynthesis Ovalbumin - genetics Plasmids Recombinant Proteins - biosynthesis Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons |
title | Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae |
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