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Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail oval...

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Published in:Folia microbiologica 1992, Vol.37 (4), p.273-278
Main Authors: KRIZKOVA, L, JELOKOVA, J, KLAUDINY, J, ZAMOCKY, M
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Language:English
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container_title Folia microbiologica
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creator KRIZKOVA, L
JELOKOVA, J
KLAUDINY, J
ZAMOCKY, M
description A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.
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subjects Animals
Biological and medical sciences
Biotechnology
Coturnix
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression - genetics
Genetic engineering
Genetic technics
Genetic Vectors
Methods. Procedures. Technologies
Ovalbumin - biosynthesis
Ovalbumin - genetics
Plasmids
Recombinant Proteins - biosynthesis
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Vectors (cloning, transfer, expression). Insertion sequences and transposons
title Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae
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