Characterization of grapefruit plants (Citrus paradisi Macf.) transformed with citrus tristeza closterovirus genes

Grapefruit (Citrus paradisi Macf. cv Duncan) plants were transformed with several sequences from citrus tristeza closterovirus (CTV) that varied in terms of position in the CTV genome and virus strain origin in an attempt to obtain resistant plants. The sequences included the capsid protein gene fro...

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Bibliographic Details
Published in:Plant cell reports 2003, Vol.21 (5), p.421-428
Main Authors: FEBRES, V. J, NIBLETT, C. L, LEE, R. F, MOORE, G. A
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Blotting, Southern
Blotting, Western
Capsid Proteins - genetics
Capsid Proteins - metabolism
Citrus paradisi - genetics
Closterovirus - genetics
DNA, Plant - genetics
Fruits
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Glucuronidase - genetics
Glucuronidase - metabolism
Pest resistance
Plant pathogens
Plants, Genetically Modified - genetics
Plasmids - genetics
RNA Replicase - genetics
RNA Replicase - metabolism
Transformation, Genetic
Transgenic plants
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:Grapefruit (Citrus paradisi Macf. cv Duncan) plants were transformed with several sequences from citrus tristeza closterovirus (CTV) that varied in terms of position in the CTV genome and virus strain origin in an attempt to obtain resistant plants. The sequences included the capsid protein gene from three different strains, a nontranslatable version of the capsid protein gene, the replicase (RdRp), the minor capsid protein (p27), a highly transcribed gene of unknown function (p20) and the more conserved 3' end of the genomic RNA. Transgenic plants were generated from all of the constructs, except from the p20 and p27 genes. Southern and Western blot analyses demonstrated that stably transformed grapefruit plants were obtained and that at least some transgenes were expressed. In a first effort at virus challenge, 25 transgenic lines were graft inoculated with a severe strain of CTV. Although some transgenic plants averaged lower titers of virus than controls, there was great variability in titer in both controls and transgenic plants, and all were apparently susceptible to the virus.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-002-0528-y