Internucleosomal chromatin degradation in myeloma and B-hybridoma cell cultures

The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c is compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isola...

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Bibliographic Details
Published in:FEBS letters 1992-11, Vol.313 (3), p.295-299
Main Authors: Sokolova, Irina A., Volgin, Andrei U., Makarova, Natalia V., Volgina, Veronica V., Shishkin, Sergei S., Khodarev, Nikolai N.
Format: Article
Language:English
Subjects:
Ageing, cell death
Animals
Apoptosis
B-Lymphocytes - metabolism
Biological and medical sciences
Ca/Mg-dependent endonuclease
Cell Cycle
Cell Death
Cell physiology
Chromatin - metabolism
DNA Damage
Endonucleases - metabolism
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Hybridoma
Hybridomas - metabolism
In Vitro Techniques
Mice
Molecular and cellular biology
Nucleosomes - metabolism
Plasmacytoma - metabolism
Tumor Cells, Cultured
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Summary:The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c is compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME, which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time—course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)81213-6