Hydrogen sulphide ameliorates ischaemia-reperfusion injury in an experimental model of non-heart-beating donor kidney transplantation

• Published in: British journal of surgery 2010-02, Vol.97 (2), p.202-209
• Main Authors: Hosgood, S. A., Nicholson, M. L.
• Format: Article
• Language: English
• Subjects: Acid-Base Equilibrium; Animals; Biological and medical sciences; Biomarkers - urine; Cardiology. Vascular system; Dinoprost - analogs & derivatives; Dinoprost - urine; Dose-Response Relationship, Drug; General aspects; Hemodynamics; Hydrogen Sulfide - therapeutic use; Kidney - blood supply; Kidney Transplantation - methods; Medical sciences; Nitric Oxide - urine; Organ Preservation - methods; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Reperfusion Injury - urine; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; Swine
• ISSN: 0007-1323; 1365-2168
• DOI: 10.1002/bjs.6856

Background: Therapies to alleviate ischaemia–reperfusion (IR) injury have an important role in kidney transplantation. This study used a porcine model of non‐heart‐beating (NHB) donor kidneys to investigate the effects of hydrogen sulphide on IR injury. Methods: Porcine kidneys were subjected to 25 min of warm ischaemia and 18 h of cold storage. They were reperfused ex vivo with autologous oxygenated blood to assess renal function. A group treated with hydrogen sulphide (0·5 mmol/l) infused 10 min before and after reperfusion (n = 6) was compared with an untreated control group (n = 7). Results: Hydrogen sulphide significantly improved renal blood flow compared with control values (mean(s.d.) area under the curve (AUC) 614·9(165·5) versus 270·3(86·7) ml per min per 100 g.h; P = 0·001) and renal function (AUC creatinine: 1640(248) versus 2328(154) µmol/l.h; P = 0·001; AUC creatinine clearance: 6·94(5·03) versus 0·96(0·32) ml per min per 100 g.h; P = 0·004). Oxidative damage was also reduced by hydrogen sulphide (urinary 8‐isoprostane at 1 h of reperfusion: 478·9(237·1) versus 1605·6(632·7) pg/ml per mmol/l creatinine; P = 0·032). Conclusion: Hydrogen sulphide ameliorated the renal dysfunction associated with ischaemic damage, and has potential as a therapy against IR injury in NHB donor kidney transplantation. Copyright © 2009 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd. Improvement of dysfunction in renal ischaemia