Does over-exposure to copper ions released from metallic copper induce cytotoxic and genotoxic effects on mammalian cells?

Abstract Background A high dissolution of copper from intrauterine devices (IUDs) occurs during the first days after insertion. This work is focused on the assessment of the possible cyto- and genotoxic effects of different concentrations of copper ions released from metallic copper on mammalian cel...

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Bibliographic Details
Published in:Contraception (Stoneham) 2010-04, Vol.81 (4), p.343-349
Main Authors: Grillo, Claudia A, Reigosa, Miguel A, Fernández Lorenzo de Mele, Mónica A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CHO Cells
Copper - toxicity
Copper ion release
Cricetinae
Cricetulus
Cytotoxicity
DNA - drug effects
DNA Damage
Female
Genital system. Reproduction
Genotoxicity
Intrauterine Devices, Copper - adverse effects
IUD
Medical sciences
Mitochondria - drug effects
Obstetrics and Gynecology
Pharmacology. Drug treatments
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Summary:Abstract Background A high dissolution of copper from intrauterine devices (IUDs) occurs during the first days after insertion. This work is focused on the assessment of the possible cyto- and genotoxic effects of different concentrations of copper ions released from metallic copper on mammalian cells in vitro. Study Design Colorimetric tetrazolium/Trypan blue (TB) tests and Comet assay were used to evaluate potential cytotoxicity and genotoxicity, respectively, in Chinese hamster ovary cells (CHO-K1). Results Reduction of mitochondrial activity by copper ions was observed for extracts at ≥7.42 mg/L concentrations, while TB exclusion test for plasma membrane integrity showed significant decrease in cell viability (close to 90%) for 10.85 mg/L concentration. Additionally, copper-induced DNA damage was detected for 5.67–7.42 mg/L concentration range. Conclusion Our results demonstrate cytotoxic and genotoxic effects of copper ions released from metallic copper on CHO-K1 cells and emphasize the importance of reducing the initial copper dissolution from IUD without affecting the contraceptive action.
ISSN:0010-7824
1879-0518
DOI:10.1016/j.contraception.2009.12.003