Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC-ESI-MS/MS

Rapid, simple and reliable HPLC/UV and LC‐ESI‐MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition...

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Bibliographic Details
Published in:Biomedical chromatography 2009-10, Vol.23 (10), p.1034-1043
Main Authors: Park, Ah Yeon, Park, So-Young, Lee, Jaehyun, Jung, Mihye, Kim, Jinwoong, Kang, Sam Sik, Youm, Jeong-Rok, Han, Sang Beom
Format: Article
Language:English
Subjects:
Angelica - chemistry
Angelicae dahuricae Radix
C30 column
Chromatography, High Pressure Liquid - methods
coumarins
Coumarins - analysis
Furans - analysis
Furocoumarins - analysis
HPLC/UV
LC-ESI-MS/MS
Linear Models
Plant Roots - chemistry
quality control
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet - methods
Tandem Mass Spectrometry - methods
Temperature
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Summary:Rapid, simple and reliable HPLC/UV and LC‐ESI‐MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition for HPLC/UV was optimized using a Develosil RPAQUEOUS C30 column using 70% acetonitrile in water as the mobile phase. This HPLC/UV method was successful for providing the baseline separation of the five coumarins with no interfering peaks detected in the 70% ethanol extract of Angelicae dahuricae Radix. The specific determination of the five coumarins was also accomplished by a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source (LC‐ESI‐MS/MS). Multiple reaction monitoring (MRM) in the positive mode was used to enhance the selectivity of detection. The LC‐ESI‐MS/MS methods were successfully applied for the determination of the five major coumarins in Angelicae dahuricae Radix. These HPLC/UV and LC‐ESI‐MS/MS methods were validated in terms of recovery, linearity, accuracy and precision (intra‐ and inter‐day validation). Taken together, the shorter analysis time involved makes these HPLC/UV and LC‐ESI‐MS/MS methods valuable for the commercial quality control of Angelicae dahuricae Radix extracts and its pharmaceutical preparations. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1219