The role of glucocorticoid in SIRPα and SHP-1 gene expression in AIHA patients

• Published in: Immunopharmacology and immunotoxicology 2009-12, Vol.31 (4), p.636-640
• Main Authors: de Almeida, Ana Carolina, Barbosa, Soraya Massaro, Lourdes Barjas-Castro, Maria de, Olalla-Saad, Sara Teresinha, Condino-Neto, Antonio
• Format: Article
• Language: English
• Subjects: Anemia, Hemolytic, Autoimmune - genetics; Anemia, Hemolytic, Autoimmune - metabolism; Antigens, Differentiation - biosynthesis; Antigens, Differentiation - genetics; Antigens, Differentiation - physiology; Autoimmune hemolytic anemia; Cells, Cultured; Dexamethasone - pharmacology; erythrophagocytosis; Gene Expression Regulation - immunology; glucocorticoid; Glucocorticoids - pharmacology; Glucocorticoids - physiology; Humans; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Phagocytosis - immunology; Protein Tyrosine Phosphatase, Non-Receptor Type 6 - biosynthesis; Protein Tyrosine Phosphatase, Non-Receptor Type 6 - genetics; Protein Tyrosine Phosphatase, Non-Receptor Type 6 - physiology; Receptors, Immunologic - biosynthesis; Receptors, Immunologic - genetics; Receptors, Immunologic - physiology; SHP-1; SIRPα
• ISSN: 0892-3973; 1532-2513
• DOI: 10.3109/08923970902939256

The aim of this work was to evaluate the regulation of SIRPα, an inhibitory phagocyte receptor, and the phosphatase SHP-1 in monocytes of patients with autoimmune hemolytic anemia, and the role of dexamethasone on SIRPα and SHP-1 gene expression and erythrophagocytosis in vitro. SIRPα and SHP-1 expression was higher in monocytes from AIHA patients compared with normal, returning to normal after glucocorticoid therapy. SIRPα and SHP-1 mRNA expression was upregulated in healthy monocytes treated with dexamethasone compared with basal; however, the erythrophagocytic ability was not altered. Our results point to a minor role of SIRPα and SHP-1 in determining AIHA.