Magnetophoretic Cell Sorting Is a Function of Antibody Binding Capacity

Antibody binding capacity (ABC) is a term representing a cellapos;s ability to bind antibodies, correlating with the number of specific cellular antigens expressed on that cell. ABC allows magnetically conjugated antibodies to bind to the targeted cells, imparting a magnetophoretic mobility on each...

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Bibliographic Details
Published in:Biotechnology progress 2003-05, Vol.19 (3), p.899-907
Main Authors: McCloskey, Kara E., Moore, Lee R., Hoyos, Mauricio, Rodriguez, Alex, Chalmers, Jeffrey J., Zborowski, Maciej
Format: Article
Language:English
Subjects:
Antibody Affinity
Biological and medical sciences
Biotechnology
Computer Simulation
Electrophoresis - instrumentation
Electrophoresis - methods
Equipment Design
Equipment Failure Analysis
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - classification
Hematopoietic Stem Cells - cytology
Humans
Immunomagnetic Separation - instrumentation
Immunomagnetic Separation - methods
Magnetics - instrumentation
Models, Biological
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Summary:Antibody binding capacity (ABC) is a term representing a cellapos;s ability to bind antibodies, correlating with the number of specific cellular antigens expressed on that cell. ABC allows magnetically conjugated antibodies to bind to the targeted cells, imparting a magnetophoretic mobility on each targeted cell. This enables sorting based on differences in the cell magnetophoretic mobility and, potentially, a magnetic separation based on the differences in the cell ABC values. A cellapos;s ABC value is a particularly important factor in continuous magnetic cell separation. This work investigates the relationship between ABC and magnetic cell separation efficiency by injection of a suspension of immunomagnetically labeled quantum simply cellular calibration microbeads of known ABC values into fluid flowing through a quadrupole magnetic sorter. The elution profiles of the outlet streams were evaluated using UV detectors. Optimal separation flow rate was shown to correlate with the ABC of these microbeads. Comparing experimental and theoretical results, the theory correctly predicted maximum separation flow rates but overestimated the separation fractional recoveries.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp020285e