Effect of environmental conditions on extracellular protease activity in lignolytic cultures of Phanerochaete chrysosporium

Two different types of extracellular protease activity were identified in the culture fluid of Phanerochaete chrysosporium wild-type BKM-F grown in submerged batch culture on N-limited media. The first activity, which appears to be inherent to the active growth phase, displayed a maximum on day 2 an...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1990-02, Vol.56 (2), p.395-400
Main Authors: Dosoretz, C.G. (Michigan State University, East Lansing, MI), Chen, H.C, Grethlein, H.E
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BASIDIOMYCETES
Biological and medical sciences
Biotechnology
BOIS
CHAMPIGNON
ENZIMAS
ENZYME
Fundamental and applied biological sciences. Psychology
FUNGI
MADERA
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Physiology and Biotechnology
PROTEASAS
PROTEASE
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Summary:Two different types of extracellular protease activity were identified in the culture fluid of Phanerochaete chrysosporium wild-type BKM-F grown in submerged batch culture on N-limited media. The first activity, which appears to be inherent to the active growth phase, displayed a maximum on day 2 and decreased to a very low level on day 4. The second activity, which appeared at day 8 following the peak of ligninase activity, seems to be characteristic of late secondary metabolism and is stimulated by carbon starvation. Cultures started with half the amount of glucose of other cultures showed a remarkably earlier development of secondary activity. In contrast, the fed-batch addition of glucose started when ligninase activity was at a maximum (day 6) completely repressed secondary protease activity and enhanced ligninase production. The addition of exogenous veratryl alcohol increased the level of secondary protease activity, whereas the oxygen supply pattern significantly affected both the time course and the level of overall proteolytic activity. The addition of phenylmethylsulfonyl fluoride to growing cultures (0, 1, or 6 days) diminished overall protease activity, while it significantly enhanced ligninase activity. In all cases, the time courses of protease and ligninase activities were negatively correlated, indicating that protease activity promotes thedecline of ligninase activity in batch culture
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.56.2.395-400.1990