Diversity among Rhizobia effective with Robinia pseudoacacia L

The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1992-07, Vol.58 (7), p.2137-2143
Main Authors: Batzli, J.M. (University of Illinois, Urbana, IL), Graves, W.R, Van Berkum, P
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biology
CARBOHIDRATOS
CRECIMIENTO
CROISSANCE
Economic plant physiology
FIJACION DEL NITROGENO
FIXATION DE L'AZOTE
Fundamental and applied biological sciences. Psychology
GLUCIDE
MEDICAMENT
MEDICAMENTOS
Microorganism-Plant Interactions
PLANTULAS
PLANTULE
PROCEDENCIA
PROVENANCE
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA QUIMICA
RHIZOBIACEAE
Rhizobium
ROBINIA PSEUDOACACIA
SIMBIOSIS
SYMBIOSE
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
TOLERANCE AU SEL
TOLERANCIA A LA SAL
Trees
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Description
Summary:The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultured, and verified to be rhizobia. The 186 isolates varied significantly in their resistance to antibiotics and NaCl, their growth on different carbohydrates, and their effect on the pH of culture media. Most isolates showed intermediate antibiotic resistance, the capacity to use numerous carbohydrates, and a neutral to acid pH response. Isolates had greater similarity within sampling locations than among sampling locations. The isolates were grouped by using numerical taxonomy techniques, and representative strains of 37 groups were selected. The mean generation times of these isolates ranged from 3 to 9 h, and the protein profile of each of the 37 isolates was unique. Nitrogen fixation, total nitrogen accumulation, and plant growth varied significantly among black locust seedlings inoculated with the representative isolates. We conclude that great variation exists among Rhizobium spp. that nodulate black locust, and selection of strains for efficiency of the symbiotic association appears possible
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.58.7.2137-2143.1992