Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes

Forty-eight strains representing the eight recognized Rhizobium species, two new Phaseolus bean Rhizobium genomic species, Bradyrhizobium spp., Agrobacterium spp. and unclassified rhizobia from various host plants were examined by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1994-01, Vol.60 (1), p.56-63
Main Authors: Laguerre, G, Allard, M.R, Revoy, F, Amarger, N
Format: Article
Language:English
Subjects:
ADN
AGROBACTERIUM RHIZOGENES
AGROBACTERIUM TUMEFACIENS
Agronomy. Soil science and plant productions
AMPLIFICATION CHAINE POLYMERASE
ARN RIBOSOMAL
ARN RIBOSOMIAL
Bacteria
Bacteriology
Biological and medical sciences
BRADYRHIZOBIUM
Cellular biology
CHIMIOTAXONOMIE
Flowers & plants
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetics
IDENTIFICACION
IDENTIFICATION
Microbiology
Microorganism-Plant Interactions
POLIMORFISMO
POLYMORPHISME
QUIMIOTAXONOMIA
REACCION DE CADENAS DE POLIMERASA
RHIZOBIUM
RIBOSOMAS
RIBOSOME
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Description
Summary:Forty-eight strains representing the eight recognized Rhizobium species, two new Phaseolus bean Rhizobium genomic species, Bradyrhizobium spp., Agrobacterium spp. and unclassified rhizobia from various host plants were examined by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes amplified by polymerase chain reaction (PCR). Twenty-one composite genotypes were obtained from the combined data of the RFLP analysis with nine endonucleases. Species assignments were in full agreement with the established taxonomic classification. Estimation from these data of genetic relationships between and within genera and species correlated well with previously published data based on DNA-rRNA hybridizations and sequence analysis of 16S rRNA genes. This PCR-RFLP method provides a rapid tool for the identification of root nodule isolates and the detection of new taxa
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.60.1.56-63.1994