Bovine colostrum or milk as a serum substitute for the cultivation of a mouse hybridoma

A mouse–mouse hybridoma was grown in serum‐free medium supplemented with bovine milk or colostrum. Bovine colostrum supported growth of the hybridoma whereas bovine milk alone did not support cellular proliferation. For growth in medium supplemented with colostrum, the maximum cell concentration ach...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1990-04, Vol.35 (9), p.882-889
Main Authors: Ramírez, O. T., Sureshkumar, G. K., Mutharasan, R.
Format: Article
Language:English
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Summary:A mouse–mouse hybridoma was grown in serum‐free medium supplemented with bovine milk or colostrum. Bovine colostrum supported growth of the hybridoma whereas bovine milk alone did not support cellular proliferation. For growth in medium supplemented with colostrum, the maximum cell concentration achieved was 1.4 × 106 cells/mL in 2.2% colostrum, which is 44% of that obtained in 9% serum. When cells were grown in media containing milk and low amounts of serum (
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260350905