The kinetic and solvent deuterium isotope effects in the 4- and 5-positions of the indole ring on the enzymatic decomposition of L-tryptophan

The kinetic and solvent deuterium isotope effects in the 4- and 5-positions of the indole ring on the enzymatic decomposition of l-tryptophan catalysed by the enzyme TPase (EC. 4.1.99.1) were determined. The isotope effects were investigated by the non-competitive method using [4′- 2 H]-l-tryptophan...

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Bibliographic Details
Published in:Isotopes in environmental and health studies 2010-06, Vol.46 (2), p.225-232
Main Authors: Winnicka, Elzbieta, Da¸browski, Pawel, Winek, Tomasz, Kanska, Marianna
Format: Article
Language:English
Subjects:
Biocatalysis
Deuterium - chemistry
enzymes
hydrogen-2
Indoles - chemistry
Indoles - metabolism
Isotope Labeling
kinetic isotope effect
Kinetics
l-tryptophan
solvent isotope effect
Solvents - chemistry
Tryptophan - metabolism
tryptophanase
Tryptophanase - metabolism
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Summary:The kinetic and solvent deuterium isotope effects in the 4- and 5-positions of the indole ring on the enzymatic decomposition of l-tryptophan catalysed by the enzyme TPase (EC. 4.1.99.1) were determined. The isotope effects were investigated by the non-competitive method using [4′- 2 H]-l-tryptophan, which was enriched in deuterium in 70% in the 4-position. The numerical values of isotope effects for 100% enrichment in deuterated label in that position were calculated by approximation. Those same isotope effects were determined for [5′- 2 H]-l-tryptophan fully deuteriated in the 5′ -position.
ISSN:1025-6016
1477-2639
DOI:10.1080/10256016.2010.488808