Immunogenicity and biophysical properties of a Nocardia brasiliensis protease involved in pathogenesis of mycetoma

We isolated and purified to homogeneity a caseinolytic protease from a Nocardia brasiliensis cell extract. Preparative polyacrylamide gel electrophoresis and electroelution were employed for purification. This purified protease was injected in BALB/c mice and induced IgM and IgG anti-protease antibo...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 2003-06, Vol.37 (1), p.37-44
Main Authors: Licón-Trillo, Ángel, Ángeles Castro-Corona, M, Salinas-Carmona, Mario César
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Antigens, Bacterial - administration & dosage
Antigens, Bacterial - immunology
Biological and medical sciences
Caseins - metabolism
Endopeptidases - administration & dosage
Endopeptidases - chemistry
Endopeptidases - immunology
Endopeptidases - isolation & purification
Female
Fundamental and applied biological sciences. Psychology
Immune Sera - immunology
Immunization, Passive
Immunogenicity
Isoelectric Focusing
Male
Mice
Mice, Inbred BALB C
Microbiology
Mycetoma
Mycetoma - prevention & control
Mycology
Nocardia - enzymology
Nocardia - pathogenicity
Nocardia brasiliensis
Nocardia Infections - prevention & control
Pathogenesis
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Protease
Vaccination
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Summary:We isolated and purified to homogeneity a caseinolytic protease from a Nocardia brasiliensis cell extract. Preparative polyacrylamide gel electrophoresis and electroelution were employed for purification. This purified protease was injected in BALB/c mice and induced IgM and IgG anti-protease antibodies. Active immunization of mice with this protease prevented mycetoma development in experimentally infected animals. Passive immunization with hyperimmune sera containing a high anti-protease antibody titer conferred partial but transient protection when collected 30 days after donor’s immunization. The protective effect of hyperimmune sera was lost when obtained from donors after 60 days from their immunization despite its higher anti-protease antibody concentration. Cytokines are good candidates to explain these findings.
ISSN:0928-8244
1574-695X
DOI:10.1016/S0928-8244(03)00102-0