Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates ( n=279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (...

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Bibliographic Details
Published in:Veterinary microbiology 2003-07, Vol.94 (3), p.257-266
Main Authors: Nauerby, B., Pedersen, K., Madsen, M.
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques - veterinary
Bacteriology
Biological and medical sciences
broiler chickens
Carrier State - veterinary
Chicken
Chickens - microbiology
clones
Clostridium perfringens
Clostridium perfringens - classification
Clostridium perfringens - genetics
Clostridium perfringens - isolation & purification
Denmark
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field - methods
Electrophoresis, Gel, Pulsed-Field - veterinary
Enteritis - microbiology
Enteritis - veterinary
Enterotoxins - genetics
farms
Fermented food industries
flocks
Food industries
Fundamental and applied biological sciences. Psychology
Genetic Variation
Microbiology
necrotic enteritis
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phylogeny
polymerase chain reaction
Poultry Diseases - microbiology
Pulsed-field gel electrophoresis
Wines and vinegars
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Summary:The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates ( n=279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(03)00118-4