DNA-specific antiidiotypic antibodies in the sera of patients with autoimmune diseases

• Published in: FEBS letters 1992-12, Vol.314 (3), p.259-263
• Main Authors: Bronshtein, Igor B., Shuster, Alexander M., Gololobov, Gennady V., Gromova, Irina I., Kvashuk, Oksana A., Belostotskaya, Ksenia M., Alekberova, Zemfira S., Prokaeva, Tatiana B., Gabibov, Alexander G.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antibodies, Anti-Idiotypic - immunology; Antibodies, Monoclonal - immunology; Antiidiotypic antibody; Arthritis, Rheumatoid - immunology; Autoantibodies - immunology; Autoimmune Diseases - immunology; Autoimmunity; Binding, Competitive; Chromatography, High Pressure Liquid; DNA - immunology; Fusion protein; high performance liquid chromatography; HPLC; HPLC of autoimmune sera; Humans; Lupus Erythematosus, Systemic - immunology; MAB; Molecular Sequence Data; monoclonal antibodies against topoisomerase; PAGE; polyacrylamide gel electrophoresis; Restriction Mapping; rheumatoid arthritis; Scl; scleroderma; Scleroderma, Systemic - immunology; SLE; systemic lupus erythematosus; Topoisomerase I
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81484-4

Blood sera of patients with autoimmune diseases scleroderma (Scl), systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA) have been shown to yield a specific immune response to topoisomerase I, the product of expression of a cDNA fragment cloned into λgt11 and monoclonal antibodies (MAB) to the enzyme. The ‘topoisomerase test’ is not absolutely specific for Scl. The stable positive response of autoimmune sera to anti-topoisomerase monoclonal antibodies has a specific character and is associated with the interaction of the Fab fragment of MAB with the IgG fraction of autoimmune serum. The response observed indicates the induction of anti-idiotypic antibodies against topoisomerase. The anti-idiotype, isolated by HPLC and affinity chromatography demonstrated the following functional activities: (i) the immunological reaction against DNA; (ii) high-affinity DNA-binding with topoisomerase-specific consensus; (iii) ability to compete with the native enzyme for binding with DNA and MAB to topoisomerase; (iv) immunological reaction against MAB to topoisomerase.