Molecular cloning and sequence analysis of an Andean potato mottle virus cDNA clone encoding the major coat protein

Andean potato mottle virus (APMV) is an endemic South American virus belonging to the comoviruses, whose host range is mainly restricted to the Solanaceae. APMV is a single-stranded positive-sense RNA virus with a bipartite genome, the so called M and B-RNA (3.5 and 5.6 kb), which are separately enc...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-09, Vol.100 (1), p.539-541
Main Authors: Shindo, N. (Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil), Brioso, P.S.T, Vicente, A.C.P, Krengiel, R, Felix, D.B, Bauw, G, Oliveira, D.E. de
Format: Article
Language:English
Subjects:
amino acid sequence
AMINO ACID SEQUENCES
ANDEAN POTATO MOTTLE COMOVIRUS
Andean potato mottle virus
Biological and medical sciences
Capsid proteins
coat protein
Codons
Comovirus
COMPLEMENTARY DNA
Fundamental and applied biological sciences. Psychology
GENBANK/M83536
genes
Genetic code
Microbiology
Molecular cloning
MOLECULAR SEQUENCE DATA
Morphology, structure, chemical composition, physicochemical properties
NUCLEOTIDE SEQUENCE
Open reading frames
Plant Gene Register
predictions
PROTEINAS
PROTEINE
PROTEINS
RNA
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Solanum tuberosum
Virology
Viruses
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Description
Summary:Andean potato mottle virus (APMV) is an endemic South American virus belonging to the comoviruses, whose host range is mainly restricted to the Solanaceae. APMV is a single-stranded positive-sense RNA virus with a bipartite genome, the so called M and B-RNA (3.5 and 5.6 kb), which are separately encapsidated into isometric particles. Here, we report the cloning and characterization of a larger cDNA from the M-RNA, which encodes, in addition to the minor CP, also the major APMV CP. Viral RNA purification, cDNA synthesis, and cloning were performed as described earlier. cDNA inserts up to 2.3 kb were isolated, assigned by northern hybridization to either APMV genomic RNA, and sequenced.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.1.539