Studies on Chlorogenic Acid Biosynthesis in Sweet Potato Root Tissue in Special Reference to the Isolation of a Chlorogenic Acid Intermediate

Marked polyphenol production takes place in root tissue of sweet potato, Ipomoea batatas Lam. cv. Norin 1, in response to slicing. A possible intermediate, tentatively termed compound V, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-14C. Comp...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1973-04, Vol.51 (4), p.768-771
Main Authors: Mineo Kojima, Uritani, Ikuzo
Format: Article
Language:English
Subjects:
Biosynthesis
Chromatography
Ethanol
Human resources
Indoleacetic acids
Paper chromatography
Plant roots
Radioactive decay
Silica gel
Sweet potatoes
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Summary:Marked polyphenol production takes place in root tissue of sweet potato, Ipomoea batatas Lam. cv. Norin 1, in response to slicing. A possible intermediate, tentatively termed compound V, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-14C. Compound V was proved to be an ester whose acid moiety was t-cinnamic acid, and the hydroxyl group-bearing moiety appeared to be a carbohydrate. Compound V was suggested to be the first intermediate after t-cinnamic acid involved in the chlorogenic acid biosynthetic pathway by the following three results. (a) label of t-cinnamic acid-2-14C was distributed in compound V first, then transferred to chlorogenic acid and isochlorogenic acid, isomers of dicaffeoylquinic acid; (b) specific radioactivity of compound V increased prior to that of the fraction containing chlorogenic acid and isochlorogenic acids and decreased prior to that of the latter; and (c) label of compound V was efficiently incorporated into chlorogenic acid and isochlorogenic acid.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.51.4.768