Incorporation of (14C) glucosamine and (14C)mannose into glycolipids and glycoproteins in cotyledons of Pisum sativum L

Developing pea cotyledons incorporate radioactivity in vivo from [14C]glucosamine and [14C]mannose into glycolipids and glycoproteins. Several different lipid components are labeled including neutral, ionicnonacidic, and acidic lipids. The acidic lipids labeled in vivo appear similar to the polyisop...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1980-05, Vol.65 (5), p.924-930
Main Authors: Browder, S.K, Beevers, L
Format: Article
Language:English
Subjects:
Acetates
Biosynthesis
carbon
Cotyledons
Glycopeptides
Glycoproteins
isotopes
Lipids
Oligosaccharides
Peas
Radioactive decay
radionuclides
Solvents
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Summary:Developing pea cotyledons incorporate radioactivity in vivo from [14C]glucosamine and [14C]mannose into glycolipids and glycoproteins. Several different lipid components are labeled including neutral, ionicnonacidic, and acidic lipids. The acidic lipids labeled in vivo appear similar to the polyisoprenoid lipid intermediates formed in vitro in pea cotyledons. Radioactivity from [14C]glucosamine and [14C]mannose is also incorporated into glycopeptides. Considerable redistribution of [14C]mannose into other glycosyl components found in endogenous glycoproteins is observed. An N-acetylglucosamine to asparagine glycopeptide linkage has been isolated from [14C]glucosamine-labeled glycoproteins.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.65.5.924