A rapid, simple method for nuclei isolation from plant [soybean] protoplasts

A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed th...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1977-08, Vol.60 (2), p.179-181
Main Authors: Ohyama, K, Pelcher, L.E, Horn, D
Format: Article
Language:English
Subjects:
Amino acids
Cell culture techniques
Centrifugation
Chemical suspensions
Cultured cells
DNA
Protoplasts
RNA
Soybeans
Tritons
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Description
Summary:A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.60.2.179