Ploidy Effects in Isogenic Populations of Alfalfa (Medicago sativa L.): IV. Similarity in Physical and Kinetic Properties of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) was isolated from isogenic diploid-tetraploid and tetraploid-octoploid sets of alfalfa (Medicago sativa L.) leaves. Molecular weights of RuBPCase subunits were similar across ploidy levels of both isogenic sets with subunits of 52,000 and 14...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1983-04, Vol.71 (4), p.966-968
Main Authors: Steven P. Meyers, Brinegar, A. Chris, Larry E. Schrader, Douglas B. Jordan, Ogren, William L.
Format: Article
Language:English
Subjects:
Alfalfa
Diploidy
Enzymes
Gels
Kinetics
Medicago sativa
Photosynthesis
Plants
Ploidies
Short Communications
Spinach
Tetraploidy
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Summary:Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) was isolated from isogenic diploid-tetraploid and tetraploid-octoploid sets of alfalfa (Medicago sativa L.) leaves. Molecular weights of RuBPCase subunits were similar across ploidy levels of both isogenic sets with subunits of 52,000 and 14,000. Apparent $K_{m}(\text{CO}_{2}$) values and substrate specificity factors ($V_{c}K_{o}/V_{o}K_{c}$) of RuBPCase were similar across ploidy levels of both isogenic sets. These results indicate that ploidy had no effect on the kinetic properties of RuBPCase in alfalfa.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.71.4.966