Effects of aspartate and other compounds on glyphosate uptake and growth inhibition in cultured carrot cells [Daucus carota, nonselective herbicides]

The strong correlation between glyphosate uptake and growth inhibition of cultured carrot (Daucus carota L. cv Danvers) cells incubated in the presence of aspartate suggests that aspartate reverses glyphosate inhibition of growth primarily by reducing intracellular glyphosate concentration. Other co...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1983-03, Vol.71 (3), p.623-626
Main Authors: Emerson D. Nafziger, Widholm, Jack M., Slife, Fred W.
Format: Article
Language:English
Subjects:
Ammonia
Cell culture techniques
Cell growth
Cultured cells
Cyclic amino acids
Flasks
Herbicides
Inoculation
Plants
Viability
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Summary:The strong correlation between glyphosate uptake and growth inhibition of cultured carrot (Daucus carota L. cv Danvers) cells incubated in the presence of aspartate suggests that aspartate reverses glyphosate inhibition of growth primarily by reducing intracellular glyphosate concentration. Other compounds which reverse glyphosate inhibition of cell growth gave a range of effects on glyphosate uptake: succinate, α-ketoglutarate, glutamate, pyruvate, and malate at 10 millimolar and phenylalanine at 2 millimolar reduced uptake by 0, 8, 11, 16, 27, and 34%, respectively. These results suggest that more than one mechanism of reversal may operate in these cells. Glyphosate and aspartate produced only minor effects on intracellular ammonia, media pH, and cell viability. This suggests that ammonia toxicity may not be an important mechanism of action of glyphosate in this system.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.71.3.623