Glycolate metabolism is under nitrogen control in Chlorella [sorokiniana, Algae, photorespiration cycles]

The utilization of nitrate and ammonia as nitrogen sources had different effects on the metabolism of glycolate in Cholorella sorokiniana. During photolithotrophic growth with nitrate as nitrogen source, glycolate was metabolized via the glycine-serine pathway. Ammonia, produced as a result of glyco...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1984-07, Vol.75 (3), p.516-520
Main Authors: Beudeker, R.F, Tabita, F.R
Format: Article
Language:English
Subjects:
Amino acids
Ammonia
Biological and medical sciences
Cell growth
Decarboxylation
Dehydrogenases
Enzymes
Fundamental and applied biological sciences. Psychology
Glycolates
Metabolism
Metabolism. Physicochemical requirements
Nitrates
Nitrogen
Photorespiration
Plant physiology and development
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Summary:The utilization of nitrate and ammonia as nitrogen sources had different effects on the metabolism of glycolate in Cholorella sorokiniana. During photolithotrophic growth with nitrate as nitrogen source, glycolate was metabolized via the glycine-serine pathway. Ammonia, produced as a result of glycolate metabolism, was reassimilated by glutamine synthetase. Two isoforms of this enzyme were present at different relative abundance in C. sorokiniana wild type and in a mutant with an increased capacity for the metabolism of glycolate (strain OR). During photolithotrophic growth in the presence of ammonia as sole nitrogen source, several lines of evidence indicated that glycolate was metabolized to malate, pyruvate, tricarboxylic acid cycle intermediates and related amino acids in C. sorokiniana wild-type cells. Malate synthase was induced and glycine decarboxylase and serine-glyoxylate aminotransferase were repressed in cells grown with ammonia. An inverse correlation was observed between aminating NADPH-glutamate dehydrogenase and the in vivo glycine decarboxylation rate.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.75.3.516