Reconstitution of the light harvesting chlorophyll a/b pigment-protein complex into developing chloroplast membranes using a dialyzable detergent

Conditions were developed to isolate the light-harvesting chlorophyll-protein complex serving photosystem II (LHC-II) using a dialyzable detergent, octylpolyoxyethylene. This LHC-II was successfully reconstituted into partially developed chloroplast thylakoids of Hordeum vulgare var Morex (barley) s...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1986-04, Vol.80 (4), p.931-937
Main Authors: Darr, S.C, Arntzen, C.J
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Barley
Biological and medical sciences
CELL STRUCTURE
Cell structures and functions
Chlorophylls
Chloroplast, photosynthetic membrane and photosynthesis
Chloroplasts
Detergents
Dialysis
Economic plant physiology
ESTRUCTURA CELULAR
Fluorescence
Fundamental and applied biological sciences. Psychology
Greening
HORDEUM VULGARE
Molecular and cellular biology
Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia)
Nutrition. Photosynthesis. Respiration. Metabolism
PIGMENT
PIGMENTOS
PIGMENTS
PLANT PROTEINS
Plants
PLASTE
PLASTIDIOS
PLASTIDS
PROTEINAS VEGETALES
PROTEINE VEGETALE
Seedlings
STRUCTURE CELLULAIRE
Thylakoids
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Summary:Conditions were developed to isolate the light-harvesting chlorophyll-protein complex serving photosystem II (LHC-II) using a dialyzable detergent, octylpolyoxyethylene. This LHC-II was successfully reconstituted into partially developed chloroplast thylakoids of Hordeum vulgare var Morex (barley) seedlings which were deficient in LHC-II. Functional association of LHC-II with the photosystem II (PSII) core complex was measured by two independent functional assays of PSII sensitization by LHC-II. A 3-fold excess of reconstituted LHC-II was required to equal the activity of LHC developing in vivo. We suggest that a linker component may be absent in the partially developed membranes which is required for specific association of the PSII core complex and LHC-II.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.80.4.931