The linamarin beta-glucosidase in Costa Rican wild lima beans (Phaseolus lunatus L.) is apoplastic

Analysis of mesophyll protoplasts and cell wall extracts of leaf discs of Costa Rican wild lima bean (Phaseolus lunatus L.) shows that the linamarase activity is confined to the apoplast. Its substrate linamarin, together with the related enzyme hydroxynitrile lyase, is found inside the cells. This...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1987-08, Vol.84 (4), p.1296-1300
Main Authors: Frehner, M, Conn, E.E
Format: Article
Language:English
Subjects:
ANALISIS
ANALYSE
ANALYSIS
Biological and medical sciences
Cell biochemistry
Cell physiology
CELL WALLS
Centrifugation
COSTA RICA
Cyanides
Enzymes
Fundamental and applied biological sciences. Psychology
GLICOSIDOS
GLUCOSIDASA
GLUCOSIDASE
GLUCOSIDASES
GLYCOSIDE
GLYCOSIDES
Leaves
LIASAS
LYASE
LYASES
Mesophyll
METABOLI046
METABOLISM
Metabolism and Enzymology
METABOLISMO
PARED CELULAR
PAROI CELLULAIRE
PHASEOLUS LUNATUS
Plant physiology and development
PLANTAS SILVESTRES
PLANTE SAUVAGE
Plants
PROTOPLASTE
PROTOPLASTO
PROTOPLASTS
Sorghum
WILD PLANTS
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Description
Summary:Analysis of mesophyll protoplasts and cell wall extracts of leaf discs of Costa Rican wild lima bean (Phaseolus lunatus L.) shows that the linamarase activity is confined to the apoplast. Its substrate linamarin, together with the related enzyme hydroxynitrile lyase, is found inside the cells. This compartmentation prevents cyanogenesis from occurring in intact tissue, and suggests that linamarin has to be protected during any translocation across the linamarase rich apoplast.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.84.4.1296