Inhibition of somatic embryogenesis in orchardgrass by endogenous cytokinins

Endogenous indoleacetic acid (IAA) and cytokinin concentrations were measured by high performance liquid chromatography in leaf sections of an orchardgrass (Dactylis glomerata L.) genotype which exhibited a high capacity for somatic embryogenesis in vitro and in two genotypes that did not exhibit th...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1988-12, Vol.88 (4), p.990-992
Main Authors: Wenck, A.R, Conger, B.V, Trigiano, R.N, Sams, C.E
Format: Article
Language:English
Subjects:
ACIDE INDOLACETIQUE
ACIDO INDOLACETICO
Biological and medical sciences
Cell culture techniques
CITOCININAS
Communications
CYTOKININE
CYTOKININS
DACTYLIS GLOMERATA
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
EMBRYONIC DEVELOPMENT
Fundamental and applied biological sciences. Psychology
GENOTIPOS
GENOTYPE
GENOTYPES
Hexanes
IAA
Insulin antibodies
Mesophyll cells
Plant cells
Plant physiology and development
Plants
Protoplasts
Somatic embryogenesis
Tissue cultures, protoplasts
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Summary:Endogenous indoleacetic acid (IAA) and cytokinin concentrations were measured by high performance liquid chromatography in leaf sections of an orchardgrass (Dactylis glomerata L.) genotype which exhibited a high capacity for somatic embryogenesis in vitro and in two genotypes that did not exhibit this capacity. The nonembryogenic genotypes contained 3- to 4-fold higher concentrations of zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, and total cytokinins than the embryogenic genotype. There were no significant differences in IAA concentrations between genotypes. Cytokinin concentrations between basal and distal sections of embryogenic genotype were not different, but the IAA concentration was significantly greater in basal sections. Somatic embryogenesis was inhibited in the embryogenic genotype by 0.001 micromolar exogenously added zeatin.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.88.4.990